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Literature summary extracted from

  • Gonzalez, E.; Fernandez, M.R.; Larroy, C.; Sola, L.; Pericas, M.A.; Pares, X.; Biosca, J.A.
    Characterization of a (2R,3R)-2,3-butaendiol dehydrogenase as the Saccharomyces cerevisiae YAL060W gene product (2000), J. Biol. Chem., 275, 35876-35885.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.4 overexpression in Saccharomyces cerevisiae Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.4 41000
-
 2 * 41000, SDS-PAGE Saccharomyces cerevisiae
1.1.1.4 81800
-
 gel filtration Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.4 Saccharomyces cerevisiae
-
-
-
1.1.1.303 Saccharomyces cerevisiae P39714 bifunctional diacetyl reductase and (R)-2,3-butanediol dehydrogenase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.4
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.4 968
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.4 (2R,3R)-2,3-butanediol + NAD+
-
 Saccharomyces cerevisiae (3R)-acetoin + NADH
-
 r
1.1.1.4 (3R,3S)-acetoin + NADH
-
 Saccharomyces cerevisiae (2R,3R)-2,3-butanediol + meso-2,3-butanediol + NAD+
-
 r
1.1.1.4 2,3-pentanediol + NAD+
-
 Saccharomyces cerevisiae 4-hydroxy-3-pentanone + 3-hydroxy-2-pentanone + NADH
-
 ?
1.1.1.4 meso-2,3-butanediol + NAD+
-
 Saccharomyces cerevisiae D-(-)-acetoin + NADH (3S)-acetoin r
1.1.1.4 additional information 3S)-2,3-butanediol, (3R/3S)-acetoin, glycerol, sorbitol or xylitol are no substrates Saccharomyces cerevisiae ?
-
 ?
1.1.1.303 diacetyl + NADH + H+ 51.4% of the (R)-2,3-butanediol dehydrogenase activity with substrate acetoin Saccharomyces cerevisiae (R)-acetoin + NAD+
-
 ir

Subunits

EC Number Subunits Comment Organism
1.1.1.4 dimer 2 * 41000, SDS-PAGE Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.4 7
-
 acetoin reduction Saccharomyces cerevisiae
1.1.1.4 8
-
 substrate oxidation Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.4 NAD+ no activity with NADP+ as cofactor Saccharomyces cerevisiae
1.1.1.303 NADH
-
 Saccharomyces cerevisiae

General Information

EC Number General Information Comment Organism
1.1.1.303 physiological function gene disruption is not lethal. Disrupted strains can grow on 2,3-butanediol, but to a lesser cell density than wild-type Saccharomyces cerevisiae