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Literature summary extracted from

  • Pavagi, S.; Kochhar, S.; Kochhar, V.K.; Sane, P.V.
    Purification and characterization of homoserine dehydrogenase from spinach leaves (1995), Biochem. Mol. Biol. Int., 36, 649-658.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.3 Calmodulin in the presence of above 0.1 mM Ca2+ Spinacia oleracea

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.3 aspartate
-
 Spinacia oleracea
1.1.1.3 EGTA
-
 Spinacia oleracea
1.1.1.3 L-cysteine
-
 Spinacia oleracea
1.1.1.3 L-threonine
-
 Spinacia oleracea
1.1.1.3 lysine
-
 Spinacia oleracea
1.1.1.3 methionine
-
 Spinacia oleracea
1.1.1.3 Trifluoperazine
-
 Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.3 0.1
-
 DL-aspartate 4-semialdehyde
-
 Spinacia oleracea
1.1.1.3 0.3
-
 NADH
-
 Spinacia oleracea
1.1.1.3 0.5
-
 L-aspartate 4-semialdehyde
-
 Spinacia oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.3 55000
-
 4 * 55000, SDS-PAGE Spinacia oleracea
1.1.1.3 220000
-
 gel filtration Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.3 Spinacia oleracea
-
 spinach
-
1.1.1.3 Spinacia oleracea
-
 also shows aspartase kinase activity
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.3
-
 Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.3 leaf
-
 Spinacia oleracea
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.3 DL-aspartate 4-semialdehyde + NADPH + H+
-
 Spinacia oleracea L-homoserine + NADP+
-
 r
1.1.1.3 L-aspartate 4-semialdehyde + NAD(P)H
-
 Spinacia oleracea L-homoserine + NAD(P)+
-
 r
1.1.1.3 L-homoserine + NAD(P)+
-
 Spinacia oleracea L-aspartate 4-semialdehyde + NAD(P)H
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.3 tetramer 4 * 55000, SDS-PAGE Spinacia oleracea