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Literature summary extracted from

  • Sainis, J.K.; Mayne, R.G.; Wallsgrove, R.M.; Lea, P.J.; Miflin, B.J.
    Localisation and characterisation of homoserine dehydrogenase isolated from barley and pea leaves (1981), Planta, 152, 491-496.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.3 L-cysteine slight inhibition of chloroplast isozyme, strong inhibition of cytoplasmic isozyme Hordeum vulgare
1.1.1.3 L-cysteine slight inhibition of chloroplast isozyme, strong inhibition of cytoplasmic isozyme Pisum sativum
1.1.1.3 L-threonine
-
 Hordeum vulgare
1.1.1.3 L-threonine
-
 Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.3 0.027
-
 NADPH isozyme II Pisum sativum
1.1.1.3 0.04
-
 aspartate 4-semialdehyde isozyme II Pisum sativum
1.1.1.3 0.046
-
 NADPH isozyme I Pisum sativum
1.1.1.3 0.066
-
 L-aspartate 4-semialdehyde isozyme I Pisum sativum
1.1.1.3 0.1
-
 L-aspartate 4-semialdehyde isozyme I Hordeum vulgare
1.1.1.3 0.36 0.4 L-aspartate 4-semialdehyde isozyme II Hordeum vulgare
1.1.1.3 0.46
-
 NADH isozyme II Pisum sativum
1.1.1.3 0.46
-
 NADH isoenzyme II, at pH 7.5, temperature not specified in the publication Pisum sativum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.3 chloroplast isozyme I Hordeum vulgare 9507
-
1.1.1.3 chloroplast isozyme I Pisum sativum 9507
-
1.1.1.3 cytoplasm isozyme II Hordeum vulgare 5737
-
1.1.1.3 cytoplasm isozyme II Pisum sativum 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.3 34000
-
 gel filtration, isozyme II, cytoplasm Pisum sativum
1.1.1.3 69000
-
 gel filtration, isozyme II, cytoplasm Hordeum vulgare
1.1.1.3 168000 174000 gel filtration, isozyme I, chloroplasts Hordeum vulgare
1.1.1.3 168000 174000 gel filtration, isozyme I, chloroplasts Pisum sativum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.3 L-aspartate 4-semialdehyde + NAD(P)H Hordeum vulgare third reaction in the pathway between aspartate and the amino acids threonine, isoleucine, methionine L-homoserine + NAD(P)+
-
 r
1.1.1.3 L-aspartate 4-semialdehyde + NAD(P)H Pisum sativum third reaction in the pathway between aspartate and the amino acids threonine, isoleucine, methionine L-homoserine + NAD(P)+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.3 Hordeum vulgare
-
 barley
-
1.1.1.3 Pisum sativum
-
 pea
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.3 separation of the two isozymes using Blue-Sepharose chromatography Pisum sativum
1.1.1.3 separation of the two isozymes using Matrex Gel Red A affinity chromatography Hordeum vulgare

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.3 leaf
-
 Hordeum vulgare
-
1.1.1.3 leaf
-
 Pisum sativum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.3 L-aspartate 4-semialdehyde + NAD(P)H
-
 Hordeum vulgare L-homoserine + NAD(P)+
-
 r
1.1.1.3 L-aspartate 4-semialdehyde + NAD(P)H
-
 Pisum sativum L-homoserine + NAD(P)+
-
 r
1.1.1.3 L-aspartate 4-semialdehyde + NAD(P)H third reaction in the pathway between aspartate and the amino acids threonine, isoleucine, methionine Hordeum vulgare L-homoserine + NAD(P)+
-
 r
1.1.1.3 L-aspartate 4-semialdehyde + NAD(P)H third reaction in the pathway between aspartate and the amino acids threonine, isoleucine, methionine Pisum sativum L-homoserine + NAD(P)+
-
 r
1.1.1.3 L-aspartate 4-semialdehyde + NADH + H+
-
 Pisum sativum L-homoserine + NAD+
-
 r
1.1.1.3 L-homoserine + NAD(P)+
-
 Hordeum vulgare L-aspartate 4-semialdehyde + NAD(P)H
-
 r
1.1.1.3 L-homoserine + NAD(P)+
-
 Pisum sativum L-aspartate 4-semialdehyde + NAD(P)H
-
 r

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.3 NADH
-
 Hordeum vulgare
1.1.1.3 NADH
-
 Pisum sativum
1.1.1.3 NADPH preferred Hordeum vulgare
1.1.1.3 NADPH preferred Pisum sativum