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Literature summary extracted from

  • Van Nedervelde, L.; Verlingen, V.; Philipp, D.; Debourg, A.
    Purificationa nd characterization of yeast 3-methyl butanal reductases involved in the removal of wort carbonyls during fermentation (1997), Proc. Congr. Eur. Brew. Conv., 26, 447-454.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.265 2-mercaptoethanol 6% inhibition at 10 mM Saccharomyces cerevisiae
1.1.1.265 Ca2+ 9% inhibition at 0.1 mM Saccharomyces cerevisiae
1.1.1.265 dithiothreitol 35% inhibition at 5 mM Saccharomyces cerevisiae
1.1.1.265 glutathione 24% inhibition at 5 mM Saccharomyces cerevisiae
1.1.1.265 KCl 16% inhibition at 0.2 M Saccharomyces cerevisiae
1.1.1.265 Mg2+ 6% inhibition at 0.1 mM Saccharomyces cerevisiae
1.1.1.265 NaCl 19% inhibition at 0.2 M Saccharomyces cerevisiae
1.1.1.265 quercetin 78% inhibition at 0.1 mM Saccharomyces cerevisiae
1.1.1.265 sodium valporate 5% inhibition at 1 mM Saccharomyces cerevisiae
1.1.1.265 Zn2+ 28% inhibition at 0.1 mM Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.265 3-methylbutanal + NAD(P)H Saccharomyces cerevisiae
-
 3-methylbutanol + NAD(P)+
-
 r
1.1.1.265 acetaldehyde + NADPH + H+ Saccharomyces cerevisiae
-
 ethanol + NADP+
-
 r
1.1.1.265 gluconate + NAD(P)H Saccharomyces cerevisiae very low activity in both reduction and oxidation D-glucose + NAD(P)+
-
 r
1.1.1.265 glyceraldehyde + NAD(P)H Saccharomyces cerevisiae
-
 glycerol + NAD(P)+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.265 Saccharomyces cerevisiae
-
 brewer's yeast
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.265 to homogeneity, chromatography techniques Saccharomyces cerevisiae

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.265 cell culture
-
 Saccharomyces cerevisiae
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.265 1.29
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.265 3-methylbutanal + NAD(P)H
-
 Saccharomyces cerevisiae 3-methylbutanol + NAD(P)+
-
 r
1.1.1.265 3-pyridine carboxaldehyde + NAD(P)H highest activity Saccharomyces cerevisiae 3-pyridinemethanol + NAD(P)+
-
 ?
1.1.1.265 acetaldehyde + NADPH + H+
-
 Saccharomyces cerevisiae ethanol + NADP+
-
 r
1.1.1.265 benzaldehyde + NAD(P)H high activity Saccharomyces cerevisiae benzyl alcohol + NAD(P)+
-
 ?
1.1.1.265 diacetyl + NAD(P)H very low activity Saccharomyces cerevisiae ? + NAD(P)+
-
 ?
1.1.1.265 furaldehyde + NAD(P)H
-
 Saccharomyces cerevisiae furfuryl alcohol + NAD(P)+
-
 ?
1.1.1.265 gluconate + NAD(P)H very low activity in both reduction and oxidation Saccharomyces cerevisiae D-glucose + NAD(P)+
-
 r
1.1.1.265 glyceraldehyde + NAD(P)H
-
 Saccharomyces cerevisiae glycerol + NAD(P)+
-
 ?
1.1.1.265 hexanal + NADPH + H+
-
 Saccharomyces cerevisiae hexanol + NADP+
-
 r
1.1.1.265 methyl glyoxal + NAD(P)H low activity Saccharomyces cerevisiae ? + NAD(P)+
-
 ?
1.1.1.265 octanal + NAD(P)H + H+
-
 Saccharomyces cerevisiae octanol + NAD(P)+
-
 ?
1.1.1.265 pentanal + NADPH
-
 Saccharomyces cerevisiae pentanol + NADP+
-
 r