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Literature summary extracted from

  • Schmitz, W.; Albers, C.; Fingerhut, R.; Conzelmann, E.
    Purification and characterization of an alpha-methylacyl-CoA racemase from human liver (1995), Eur. J. Biochem., 231, 815-822.
    View publication on PubMed

Application

EC Number Application Comment Organism
5.1.99.4 medicine the activity of EC 5.1.99.4 may prove to be a valuable parameter for the prenatal diagnosis of general defects of peroxisome biogenesis such as Zellweger syndrome Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.99.4 2-(4-Isobutylphenyl)propionic acid
-
 Homo sapiens
5.1.99.4 2-(4-Isobutylphenyl)propionic acid
-
 Rattus norvegicus
5.1.99.4 2-methylmyristoyl-CoA
-
 Homo sapiens
5.1.99.4 2-methylmyristoyl-CoA
-
 Rattus norvegicus
5.1.99.4 2-methyloctanoyl-CoA
-
 Homo sapiens
5.1.99.4 2-methyloctanoyl-CoA
-
 Rattus norvegicus
5.1.99.4 palmitoyl-CoA inhibition is caused by the formation of stable mixed micelles Homo sapiens
5.1.99.4 palmitoyl-CoA
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.99.4 0.0316
-
 trihydroxycoprostanoyl-CoA
-
 Homo sapiens
5.1.99.4 0.172
-
 pristanoyl-CoA
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.1.99.4 mitochondrion co-distributed exclusively with mitochondrial marker enzymes Rattus norvegicus 5739
-
5.1.99.4 mitochondrion only 10-30% of the activity is found in mitochondria Homo sapiens 5739
-
5.1.99.4 peroxisome bulk activity Rattus norvegicus 5777
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.99.4 47100
-
 1 * 47100, SDS-PAGE Homo sapiens
5.1.99.4 47700
-
 gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
5.1.99.4 Homo sapiens
-
-
-
5.1.99.4 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.99.4
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.1.99.4 chorionic villus
-
 Homo sapiens
-
5.1.99.4 fibroblast
-
 Homo sapiens
-
5.1.99.4 Hep-G2 cell
-
 Homo sapiens
-
5.1.99.4 liver
-
 Homo sapiens
-
5.1.99.4 liver
-
 Rattus norvegicus
-
5.1.99.4 skin fibroblast
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.99.4 (S)-2-Methyltetradecanoyl-CoA r Homo sapiens (R)-2-Methyltetradecanoyl-CoA
-
 ?
5.1.99.4 (S)-Pristanoyl-CoA
-
 Homo sapiens (R)-Pristanoyl-CoA
-
 ?
5.1.99.4 (S)-Pristanoyl-CoA r Rattus norvegicus (R)-Pristanoyl-CoA
-
 ?
5.1.99.4 (S)-Trihydroxycoprostanoyl-CoA
-
 Homo sapiens (R)-Trihydroxycoprostanoyl-CoA
-
 ?
5.1.99.4 (S)-Trihydroxycoprostanoyl-CoA
-
 Rattus norvegicus (R)-Trihydroxycoprostanoyl-CoA
-
 ?
5.1.99.4 additional information
-
 Rattus norvegicus ?
-
 ?
5.1.99.4 additional information no activity with free fatty acids Homo sapiens ?
-
 ?
5.1.99.4 pristanoyl-CoA
-
 Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.1.99.4 monomer
-
 Rattus norvegicus
5.1.99.4 monomer 1 * 47100, SDS-PAGE Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.1.99.4 35 40 slow loss of activity Homo sapiens
5.1.99.4 50
-
 half-life: 15 min Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.99.4 8
-
-
 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.1.99.4 6.5 9 more than 80% of maximal activity between pH 6.5 and pH 9.0, inactive below pH 5 Homo sapiens