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Literature summary extracted from

  • Schmitz, W.; Fingerhut, R.; Conzelmann, E.
    Purification and properties of an alpha-methylacyl-CoA racemase from rat liver (1994), Eur. J. Biochem., 222, 313-323.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.1.99.4 palmitoyl-CoA stimulates at low concentrations, inhibits above 0.1 mM Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.99.4 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide
-
 Rattus norvegicus
5.1.99.4 2-(4-Isobutylphenyl)propionic acid i.e. ibuprofen, strongest of all competitive inhibitors tested Rattus norvegicus
5.1.99.4 2-methylmyristoyl-CoA inhibits reaction with 2-pristanoyl-CoA Rattus norvegicus
5.1.99.4 2-methyloctanoyl-CoA inhibits reaction with 2-pristanoyl-CoA Rattus norvegicus
5.1.99.4 5,5'-dithiobis(2-nitrobenzoate) inhibition is reversed by incubation of the inactivated enzyme with 10 mM dithiothreitol Rattus norvegicus
5.1.99.4 Cu2+
-
 Rattus norvegicus
5.1.99.4 diethylpyrocarbonate
-
 Rattus norvegicus
5.1.99.4 diisopropylphosphofluoridate
-
 Rattus norvegicus
5.1.99.4 Fe2+ slight inhibition Rattus norvegicus
5.1.99.4 Hg2+
-
 Rattus norvegicus
5.1.99.4 additional information no inhibition by iodoacetamide Rattus norvegicus
5.1.99.4 NEM weak Rattus norvegicus
5.1.99.4 palmitoyl-CoA stimulates at low concentrations, inhibits above 0.1 mM Rattus norvegicus
5.1.99.4 thimerosal slight Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.99.4 0.06
-
 trihydroxycoprostanoyl-CoA
-
 Rattus norvegicus
5.1.99.4 0.076
-
 pristanoyl-CoA
-
 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.99.4 44700
-
 gel filtration Rattus norvegicus
5.1.99.4 44900
-
 1 * 44900, SDS-PAGE Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
5.1.99.4 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.99.4
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.1.99.4 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.1.99.4 additional information
-
 development of a very sensitive and convenient radiometric assay Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.99.4 (S)-2-Methylmyristoyl-CoA r Rattus norvegicus (R)-2-Methylmyristoyl-CoA
-
 ?
5.1.99.4 (S)-Pristanoyl-CoA r Rattus norvegicus (R)-Pristanoyl-CoA
-
 ?
5.1.99.4 (S)-Trihydroxycoprostanoyl-CoA r Rattus norvegicus (R)-Trihydroxycoprostanoyl-CoA
-
 ?
5.1.99.4 additional information no activity with 3-methyl-branched or linear-chain acyl-CoAs Rattus norvegicus ?
-
 ?
5.1.99.4 pristanoyl-CoA
-
 Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.1.99.4 monomer 1 * 44900, SDS-PAGE Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.99.4 6
-
 pristanoyl-CoA Rattus norvegicus
5.1.99.4 7
-
 trihydroxycoprostanoyl-CoA Rattus norvegicus