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Literature summary extracted from
- DL-Methylmalonyl-CoA racemase from rat liver (1988), Methods Enzymol., 166, 400-406.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.1.99.1 | Cd2+ | - |
Rattus norvegicus |  |
| 5.1.99.1 | Cu2+ | - |
Rattus norvegicus | |
| 5.1.99.1 | EDTA | reactivation by Co2+ | Rattus norvegicus | |
| 5.1.99.1 | Zn2+ | - |
Rattus norvegicus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.1.99.1 | 0.1 | - |
DL-methylmalonyl-CoA | - |
Rattus norvegicus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.1.99.1 | Co2+ | increases activity | Rattus norvegicus | |
| 5.1.99.1 | Co3+ | lower level of activation than Co2+ at higher concentrations | Rattus norvegicus | |
| 5.1.99.1 | Fe2+ | lower level of activation than Co2+ at higher concentrations | Rattus norvegicus | |
| 5.1.99.1 | Mn2+ | lower level of activation than Co2+ at higher concentrations | Rattus norvegicus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.1.99.1 | 16000 | - |
2 * 16000, subunits not connected by disulfide bonds, SDS-PAGE in presence and absence of 2-mercaptoethanol | Rattus norvegicus |
| 5.1.99.1 | 32000 | - |
gel filtration | Rattus norvegicus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.1.99.1 | Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.1.99.1 | - |
Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 5.1.99.1 | liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.1.99.1 | 8400 | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.1.99.1 | (R)-2-Methyl-3-oxopropanoyl-CoA | - |
Rattus norvegicus | (S)-2-Methyl-3-oxopropanoyl-CoA | - |
? | |
| 5.1.99.1 | DL-methylmalonyl-CoA | - |
Rattus norvegicus | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.1.99.1 | dimer | 2 * 16000, subunits not connected by disulfide bonds, SDS-PAGE in presence and absence of 2-mercaptoethanol | Rattus norvegicus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.1.99.1 | 4170 | - |
DL-methylmalonyl-CoA | - |
Rattus norvegicus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.1.99.1 | 7 | - |
- |
Rattus norvegicus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.1.99.1 | 5 | 9 | 50% of maximal activity at pH 5.5 and 8.5 | Rattus norvegicus |
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