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Literature summary extracted from
- Purification and some properties of two acid ribonucleases from the mycelia of Aspergillus niger (1980), J. Gen. Appl. Microbiol., 26, 375-385.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.6.1.19 | Cu2+ | - |
Aspergillus niger |  |
| 4.6.1.19 | Hg2+ | - |
Aspergillus niger | |
| 4.6.1.24 | Fe3+ | Aspergillus niger RNase II | Aspergillus niger | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.6.1.19 | KH2PO4 | slight activation | Aspergillus niger | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.6.1.19 | 45000 | - |
gel filtration | Aspergillus niger |
| 4.6.1.24 | 13000 | - |
Aspergillus niger, RNase II, gel filtration | Aspergillus niger |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.6.1.19 | Aspergillus niger | - |
- |
- |
| 4.6.1.24 | Aspergillus niger | - |
similar enzyme | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.6.1.19 | - |
Aspergillus niger |
| 4.6.1.24 | similar enzyme, partial | Aspergillus niger |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 4.6.1.24 | mycelium | - |
Aspergillus niger | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.6.1.19 | additional information | - |
- |
Aspergillus niger |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.6.1.19 | RNA + H2O | - |
Aspergillus niger | 3'-phosphomononucleotides | - |
? | |
| 4.6.1.24 | RNA + H2O | - |
Aspergillus niger | Hydrolyzed RNA | 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp | ? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.6.1.19 | 60 | - |
- |
Aspergillus niger |
| 4.6.1.24 | 50 | - |
Aspergillus niger RNase II | Aspergillus niger |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.6.1.19 | 40 | 80 | 40°C, 80°C: less than 15% of maximal activity above and below | Aspergillus niger |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.6.1.19 | 3.5 | - |
- |
Aspergillus niger |
| 4.6.1.24 | 4.5 | - |
Aspergillus niger RNase II | Aspergillus niger |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.6.1.24 | 3 | 6 | 3: 25% of activity maximum, 6: about 10% of activity maximum, Aspergillus niger RNase II | Aspergillus niger |
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