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Literature summary extracted from

  • Ludmerer, S.W.; Wright, D.J.; Schimmel, P.
    Purification of glutamine tRNA synthetase from Saccharomyces cerevisiae (1993), J. Biol. Chem., 268, 5519-5523.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.18 additional information truncated enzyme form lacking the NH2-terminal domain, with modest increase in Km value for glutamine and ATP and no difference in kcat for aminoacylation or Km for tRNA Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.18 0.0017
-
 tRNAGln full-length enzyme and truncated enzyme Saccharomyces cerevisiae
6.1.1.18 0.028
-
 Gln full-length enzyme Saccharomyces cerevisiae
6.1.1.18 0.038
-
 ATP full-length enzyme Saccharomyces cerevisiae
6.1.1.18 0.11
-
 Gln truncated enzyme Saccharomyces cerevisiae
6.1.1.18 0.26
-
 ATP truncated enzyme Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.18 91000
-
 1 * 91000, SDS-PAGE Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.18 Saccharomyces cerevisiae
-
 full-length and truncated enzyme form lacking the NH2-terminal domain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.18
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.18 additional information
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.18 ATP + L-glutamine + tRNAGln
-
 Saccharomyces cerevisiae AMP + diphosphate + L-glutaminyl-tRNAGln
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.18 monomer 1 * 91000, SDS-PAGE Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.18 additional information
-
 additional information
-
 Saccharomyces cerevisiae