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Literature summary extracted from

  • Yuan, L.; Voelker, T.A.; Hawkins, D.J.
    Modification of the substrate specificity of an acyl-acyl carrier protein thioesterase by protein engineering (1995), Proc. Natl. Acad. Sci. USA, 92, 10639-10643.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.21 expression in Escherichia coli, and in developing oilseeds of Arabidopsis thaliana and Brassica napus Umbellularia californica

Protein Variants

EC Number Protein Variants Comment Organism
3.1.2.21 M197E/E199H the double mutant M197R/R199H has equal preference for dodecanoyl-[acyl-carrier protein] and tetradecanoyl-[acyl-carrier protein]. Mutant T231K shows no altered specificity. Mutant R327Q and R322M/R327Q have specific activities towards dodecanoyl-[acyl-carrier protein] and tetradecanoyl-[acyl-carrier protein] decreased about 100fold and 30fold. The triple mutant M197R/R199H/T231K is a tetradecanoyl-[acyl-carrier protein] thioesterase Umbellularia californica

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.21 dodecanoyl-[acyl-carrier protein] competitive with respect to tetradecanoyl-[acyl-carrier protein], wild type enzyme and mutant enzyme M197R/R199H/T123K Umbellularia californica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.21 0.0019
-
 dodecanoyl-[acyl-carrier protein] wild type enzyme Umbellularia californica
3.1.2.21 0.0023
-
 tetradecanoyl-[acyl-carrier protein] triple mutant M197R/R199H/T231K Umbellularia californica
3.1.2.21 0.0064
-
 tetradecanoyl-[acyl-carrier protein] wild type enzyme Umbellularia californica

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.21 Umbellularia californica
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.21 dodecanoyl-[acyl-carrier protein] + H2O the C-terminal two-third of the protein is critical for the specificity Umbellularia californica [acyl-carrier protein] + dodecanoate
-
 ?
3.1.2.21 tetradecanoyl-[acyl-carrier protein] + H2O the C-terminal two-third of the protein is critical for the specificity Umbellularia californica [acyl-carrier protein] + tetradecanoate
-
 ?