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Literature summary extracted from

  • Egana, L.; Gutierrez, R.; Caputo, V.; Peirano, A.; Steiner, J.; Eyzaguirre, J.
    Purification and characterization of two acetyl xylan esterases from Penicillium purpurogenum (1996), Biotechnol. Appl. Biochem., 24, 33-39.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.72 extracellular
-
 Talaromyces purpureogenus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.6 23000
-
 ? * 23000, SDS-PAGE, AXE II Talaromyces purpureogenus
3.1.1.6 48000
-
 ? * 48000, SDS-PAGE, AXE I Talaromyces purpureogenus
3.1.1.72 23000
-
 x * 23000, isoenzyme AXE II, SDS-PAGE Talaromyces purpureogenus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.6 Talaromyces purpureogenus
-
-
-
3.1.1.72 Talaromyces purpureogenus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.6 two enzymes: AXE I and AXE II Talaromyces purpureogenus
3.1.1.72 2 isoenzymes: AXE I and AXE II Talaromyces purpureogenus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.6 118.1
-
 AXE I Talaromyces purpureogenus
3.1.1.6 139.8
-
 AXE II Talaromyces purpureogenus
3.1.1.72 118.1
-
 isoenzyme AXE I Talaromyces purpureogenus
3.1.1.72 139.8
-
 isoenzyme AXE II Talaromyces purpureogenus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.6 acetylxylan + H2O
-
 Talaromyces purpureogenus ?
-
 ?
3.1.1.6 alpha-naphthyl acetate + H2O
-
 Talaromyces purpureogenus alpha-naphthol + acetate
-
 ?
3.1.1.72 acetylxylan
-
 Talaromyces purpureogenus acetate + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.6 More ? * 23000, SDS-PAGE, AXE II Talaromyces purpureogenus
3.1.1.6 More ? * 48000, SDS-PAGE, AXE I Talaromyces purpureogenus
3.1.1.72 ? x * 23000, isoenzyme AXE II, SDS-PAGE Talaromyces purpureogenus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.6 50
-
 AXE I Talaromyces purpureogenus
3.1.1.6 60
-
 AXE II Talaromyces purpureogenus
3.1.1.72 50
-
 isoenzyme AXE I Talaromyces purpureogenus
3.1.1.72 55 60 isoenzyme AXE II Talaromyces purpureogenus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.72 42
-
 1 h, isoenzyme AXE I: 80% loss of activity Talaromyces purpureogenus
3.1.1.72 65
-
 1 h, isoenzyme AXE II: 80% loss of activity Talaromyces purpureogenus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.6 5.3
-
 AXE I Talaromyces purpureogenus
3.1.1.6 6
-
 AXE II Talaromyces purpureogenus
3.1.1.72 5.3
-
 isoenzyme AXE I Talaromyces purpureogenus
3.1.1.72 5.5 6 isoenzyme AXE II Talaromyces purpureogenus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.72 4
-
 4°C, 24 h, isoenzyme II, stable Talaromyces purpureogenus
3.1.1.72 4 8 4°C, 24 h, isoenzyme I, stable Talaromyces purpureogenus