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Literature summary extracted from

  • Sundberg, M.; Poutanen, K.
    Purification and properties of two acetylxylan esterases of Trichoderma reesei (1991), Biotechnol. Appl. Biochem., 13, 1-11.
No PubMed abstract available

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.72 20000
-
 gel filtration Trichoderma reesei

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.72 Trichoderma reesei
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.72 glycoprotein isoenzyme AXE I and AXE II contain 12-15% carbohydrate, 60% of the carbohydrate is mannose. At least a part of the carbohydrate is N-linked. The enzymes probably also contain O-linked carbohydrates which are not released by endoglycosidase H treatment Trichoderma reesei

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.72 2 isoenzymes: AXE I and AXE II Trichoderma reesei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.72 additional information
-
-
 Trichoderma reesei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.72 4-nitrophenyl acetate + H2O
-
 Trichoderma reesei 4-nitrophenol + acetate
-
 ?
3.1.1.72 alpha-naphthyl acetate
-
 Trichoderma reesei acetate + 2-naphthol
-
 ?
3.1.1.72 glucose pentaacetate
-
 Trichoderma reesei acetate + ?
-
 ?
3.1.1.72 xylose tetraacetate
-
 Trichoderma reesei acetate + ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.72 60 65
-
 Trichoderma reesei

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.72 45
-
 pH 3-7, 24 h, stable Trichoderma reesei
3.1.1.72 60
-
 pH 5, stable up to 60°C Trichoderma reesei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.72 5 6
-
 Trichoderma reesei

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.72 3 7 45°C, 24 h, stable Trichoderma reesei
3.1.1.72 5
-
 stable up to 60°C Trichoderma reesei