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Literature summary extracted from

  • Ding, L.; Seto, B.L.; Ahmed, S.A.; Coleman, W.G.
    Purification and properties of the Escherichia coli K-12 NAD-dependent nucleotide diphosphosulfate epimerase, ADP-L-glycero-D-mannoheptose 6-epimerase (1994), J. Biol. Chem., 269, 24384-24390.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.3.20 ADP
-
Escherichia coli
5.1.3.20 ADPglucose
-
Escherichia coli
5.1.3.20 ATP
-
Escherichia coli
5.1.3.20 NADH
-
Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.3.20 0.1
-
ADP-D-glycero-D-manno-heptose
-
Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.3.20 37000
-
6 * 37000, SDS-PAGE Escherichia coli
5.1.3.20 230000 250000 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.3.20 ADP-D-glycero-D-manno-heptose Escherichia coli the enzyme is involved in the biosynthesis of ADP-L-glycero-D-mannoheptose, which is a key intermediate of lipopolysaccharide inner core ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.20 Escherichia coli
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
5.1.3.20 glycoprotein
-
Escherichia coli

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.3.20
-
Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.1.3.20 0.00087
-
-
Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.20 ADP-D-glycero-D-manno-heptose
-
Escherichia coli ADP-L-glycero-D-manno-heptose
-
?
5.1.3.20 ADP-D-glycero-D-manno-heptose the enzyme is involved in the biosynthesis of ADP-L-glycero-D-mannoheptose, which is a key intermediate of lipopolysaccharide inner core Escherichia coli ?
-
?

Subunits

EC Number Subunits Comment Organism
5.1.3.20 hexamer 6 * 37000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.3.20 42
-
-
Escherichia coli

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.1.3.20 5 55 5°C: about 45% of maximal activity, 55°C: about 50% of maximal activity Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.3.20 5.5 9.5
-
Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
5.1.3.20 NAD+ contains 1 mol of tightly bound NAD+ per mol of enzyme subunit Escherichia coli