BRENDA - Enzyme Database

Purification and properties of the Escherichia coli K-12 NAD-dependent nucleotide diphosphosulfate epimerase, ADP-L-glycero-D-mannoheptose 6-epimerase

Ding, L.; Seto, B.L.; Ahmed, S.A.; Coleman, W.G.; J. Biol. Chem. 269, 24384-24390 (1994)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
5.1.3.20
ADP
-
Escherichia coli
5.1.3.20
ADPglucose
-
Escherichia coli
5.1.3.20
ATP
-
Escherichia coli
5.1.3.20
NADH
-
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.1.3.20
0.1
-
ADP-D-glycero-D-manno-heptose
-
Escherichia coli
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.1.3.20
37000
-
6 * 37000, SDS-PAGE
Escherichia coli
5.1.3.20
230000
250000
gel filtration
Escherichia coli
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.1.3.20
ADP-D-glycero-D-manno-heptose
Escherichia coli
the enzyme is involved in the biosynthesis of ADP-L-glycero-D-mannoheptose, which is a key intermediate of lipopolysaccharide inner core
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.1.3.20
Escherichia coli
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
5.1.3.20
glycoprotein
-
Escherichia coli
Purification (Commentary)
EC Number
Commentary
Organism
5.1.3.20
-
Escherichia coli
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
5.1.3.20
0.00087
-
-
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.1.3.20
ADP-D-glycero-D-manno-heptose
-
2357
Escherichia coli
ADP-L-glycero-D-manno-heptose
-
2357
Escherichia coli
-
5.1.3.20
ADP-D-glycero-D-manno-heptose
the enzyme is involved in the biosynthesis of ADP-L-glycero-D-mannoheptose, which is a key intermediate of lipopolysaccharide inner core
2357
Escherichia coli
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
5.1.3.20
hexamer
6 * 37000, SDS-PAGE
Escherichia coli
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
5.1.3.20
42
-
-
Escherichia coli
Temperature Range [C]
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
5.1.3.20
5
55
5C: about 45% of maximal activity, 55C: about 50% of maximal activity
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.1.3.20
5.5
9.5
-
Escherichia coli
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
5.1.3.20
NAD+
contains 1 mol of tightly bound NAD+ per mol of enzyme subunit
Escherichia coli
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
5.1.3.20
NAD+
contains 1 mol of tightly bound NAD+ per mol of enzyme subunit
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
5.1.3.20
ADP
-
Escherichia coli
5.1.3.20
ADPglucose
-
Escherichia coli
5.1.3.20
ATP
-
Escherichia coli
5.1.3.20
NADH
-
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.1.3.20
0.1
-
ADP-D-glycero-D-manno-heptose
-
Escherichia coli
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.1.3.20
37000
-
6 * 37000, SDS-PAGE
Escherichia coli
5.1.3.20
230000
250000
gel filtration
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.1.3.20
ADP-D-glycero-D-manno-heptose
Escherichia coli
the enzyme is involved in the biosynthesis of ADP-L-glycero-D-mannoheptose, which is a key intermediate of lipopolysaccharide inner core
?
-
-
-
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
5.1.3.20
glycoprotein
-
Escherichia coli
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.1.3.20
-
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
5.1.3.20
0.00087
-
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.1.3.20
ADP-D-glycero-D-manno-heptose
-
2357
Escherichia coli
ADP-L-glycero-D-manno-heptose
-
2357
Escherichia coli
-
5.1.3.20
ADP-D-glycero-D-manno-heptose
the enzyme is involved in the biosynthesis of ADP-L-glycero-D-mannoheptose, which is a key intermediate of lipopolysaccharide inner core
2357
Escherichia coli
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
5.1.3.20
hexamer
6 * 37000, SDS-PAGE
Escherichia coli
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
5.1.3.20
42
-
-
Escherichia coli
Temperature Range [C] (protein specific)
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
5.1.3.20
5
55
5C: about 45% of maximal activity, 55C: about 50% of maximal activity
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.1.3.20
5.5
9.5
-
Escherichia coli