EC Number | Application | Comment | Organism |
---|---|---|---|
5.1.3.2 | synthesis | preparation of a fusion enzyme consisting of UDP-galactose 4-epimerase and galactose-1-phosphate uridylyltransferase with an intervening Ala3 linker, shows kinetic advantages in that the initial velocity to produce glucose 1-phosphate from UDPgalactose an | Escherichia coli |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.7.12 | construction and overexpression of bifunctional fusion protein composed of galactose-2-phosphate uridylyltransferase and UDP-galactose 4-epimerase with an intervening linker of 3 Ala residues | Escherichia coli |
5.1.3.2 | fusion enzyme consisting of UDP-galactose 4-epimerase and galactose-1-phosphate uridylyltransferase with an intervening Ala3 linker | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.7.12 | additional information | construction and expression of bifunctional fusion protein composed of galactose-2-phosphate uridylyltransferase and UDP-galactose 4-epimerase with an intervening linker of 3 Ala residues, 20% increased Vmax compared to a mixture of the single enzymes | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.12 | 0.08 | - |
UDP-glucose | purified recombinant bifunctional chimeric fusion protein, pH 8.5, 27°C | Escherichia coli | |
2.7.7.12 | 0.29 | - |
alpha-D-galactose 1-phosphate | purified recombinant bifunctional chimeric fusion protein, pH 8.5, 27°C | Escherichia coli | |
5.1.3.2 | 0.14 | - |
UDPgalactose | fusion enzyme consisting of UDP-galactose 4-epimerase and galactose-1-phosphate uridylyltransferase with an intervening Ala3 linker | Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.7.12 | additional information | - |
the recombinant chimeric fusion protein exists as monomer, dimer and tetramer, all are active | Escherichia coli |
2.7.7.12 | 80000 | - |
recombinant bifunctional chimeric fusion protein, monomer, gel filtration | Escherichia coli |
5.1.3.2 | 80000 | - |
2 * 80000, fusion enzyme consisting of UDP-galactose 4-epimerase and galactose-1-phosphate uridylyltransferase with an intervening Ala3 linker exist as monomeric, dimeric and tetrameric form. The monomeric form has low epimerase activity, SDS-PAGE | Escherichia coli |
5.1.3.2 | 80000 | - |
4 * 80000, fusion enzyme consisting of UDP-galactose 4-epimerase and galactose-1-phosphate uridylyltransferase with an intervening Ala3 linker exist as monomeric, dimeric and tetrameric form. The monomeric form has low epimerase activity, SDS-PAGE | Escherichia coli |
5.1.3.2 | 160000 | - |
fusion enzyme consisting of UDP-galactose 4-epimerase and galactose-1-phosphate uridylyltransferase with an intervening Ala3 linker, dimeric form, gel filtration | Escherichia coli |
5.1.3.2 | 320000 | - |
fusion enzyme consisting of UDP-galactose 4-epimerase and galactose-1-phosphate uridylyltransferase with an intervening Ala3 linker, tetrameric form, gel filtration | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.7.12 | Escherichia coli | - |
galT gene | - |
5.1.3.2 | Escherichia coli | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.7.12 | recombinant bifunctional fusion protein from strain BL21(DE3), pLysS, pT7ET to near homogeneity, 9fold | Escherichia coli |
5.1.3.2 | fusion enzyme consisting of UDP-galactose 4-epimerase and galactose-1-phosphate uridylyltransferase with an intervening Ala3 linker | Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.7.12 | 1080 | - |
purified recombinant bifunctional chimeric fusion protein | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.12 | UDP-glucose + alpha-D-galactose 1-phosphate | recombinant bifunctional chimeric fusion protein | Escherichia coli | alpha-D-glucose 1-phosphate + UDP-galactose | - |
r | |
5.1.3.2 | UDP-galactose | - |
Escherichia coli | UDP-glucose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.7.12 | More | the recombinant chimeric fusion protein exists as monomer, dimer and tetramer, all are active | Escherichia coli |
5.1.3.2 | dimer | 2 * 80000, fusion enzyme consisting of UDP-galactose 4-epimerase and galactose-1-phosphate uridylyltransferase with an intervening Ala3 linker exist as monomeric, dimeric and tetrameric form. The monomeric form has low epimerase activity, SDS-PAGE | Escherichia coli |
5.1.3.2 | monomer | 2 * 80000, fusion enzyme consisting of UDP-galactose 4-epimerase and galactose-1-phosphate uridylyltransferase with an intervening Ala3 linker exist as monomeric, dimeric and tetrameric form. The monomeric form has low epimerase activity, SDS-PAGE | Escherichia coli |
5.1.3.2 | tetramer | 4 * 80000, fusion enzyme consisting of UDP-galactose 4-epimerase and galactose-1-phosphate uridylyltransferase with an intervening Ala3 linker exist as monomeric, dimeric and tetrameric form. The monomeric form has low epimerase activity, SDS-PAGE | Escherichia coli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.12 | 24 | - |
alpha-D-galactose 1-phosphate | purified recombinant bifunctional chimeric fusion protein, pH 8.5, 27°C | Escherichia coli | |
5.1.3.2 | 960 | - |
UDPgalactose | fusion enzyme consisting of UDP-galactose 4-epimerase and galactose-1-phosphate uridylyltransferase with an intervening Ala3 linker | Escherichia coli |