BRENDA - Enzyme Database

Purification and partial characterization of a glutamyl-tRNA synthetase from the unicellular green alga Scenedesmus obliquus, mutant C-2A'

Vothknecht, U.C.; Senger, H.; Dörnemann, D.; Planta 192, 256-260 (1994)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.1.1.17
0.0023
-
Glu
-
Tetradesmus obliquus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.1.1.17
55000
-
2 * 55000, SDS-PAGE
Tetradesmus obliquus
6.1.1.17
105000
-
gel filtration
Tetradesmus obliquus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.1.1.17
Tetradesmus obliquus
-
yellow pigment mutant C-2A'
-
Purification (Commentary)
EC Number
Commentary
Organism
6.1.1.17
-
Tetradesmus obliquus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.1.1.17
additional information
-
-
Tetradesmus obliquus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGlu
charges tRNAGlu from barley and from E. coli
231
Tetradesmus obliquus
AMP + diphosphate + L-glutamyl-tRNAGlu
-
231
Tetradesmus obliquus
-
Subunits
EC Number
Subunits
Commentary
Organism
6.1.1.17
dimer
2 * 55000, SDS-PAGE
Tetradesmus obliquus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.1.1.17
8
-
-
Tetradesmus obliquus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
6.1.1.17
7
9
-
Tetradesmus obliquus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.1.1.17
0.0023
-
Glu
-
Tetradesmus obliquus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.1.1.17
55000
-
2 * 55000, SDS-PAGE
Tetradesmus obliquus
6.1.1.17
105000
-
gel filtration
Tetradesmus obliquus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.1.1.17
-
Tetradesmus obliquus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.1.1.17
additional information
-
-
Tetradesmus obliquus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGlu
charges tRNAGlu from barley and from E. coli
231
Tetradesmus obliquus
AMP + diphosphate + L-glutamyl-tRNAGlu
-
231
Tetradesmus obliquus
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
6.1.1.17
dimer
2 * 55000, SDS-PAGE
Tetradesmus obliquus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.1.1.17
8
-
-
Tetradesmus obliquus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
6.1.1.17
7
9
-
Tetradesmus obliquus