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Literature summary extracted from

  • Campbell, P.; Hannesson, H.H.; Sandbäck, D.; Roden, L.; Lindahl, U.; Li, J.
    Biosynthesis of heparin/heparan sulfate. Purification of the D-glucuronyl C-5 epimerase from bovine liver (1994), J. Biol. Chem., 269, 26953-26958.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.3.17 KCl stimulates, maximal effect at 0.1 M, inhibition at higher concentrations Bos taurus
5.1.3.17 NaCl stimulates, maximal effect at 0.05 M, inhibition at higher concentrations Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.3.17 0.063
-
 uronic acid residues
-
 Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.1.3.17 additional information no specific metal ion requirement Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.3.17 52000
-
 x * 52000, liver, SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.3.17 additional information Bos taurus enzyme is involved in the biosynthesis of heparan sulfate in liver ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.17 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.3.17
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.1.3.17 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.1.3.17 additional information
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
5.1.3.17 4°C, 10% glycerol, retains full activity for 1.5 years Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.17 Heparosan-N-sulfate D-glucuronate heparosan N-sulfate prepared by metabolic labeling of a capsular polysaccharide from E. coli K5 and subsequent chemical partial N-deacetylation and N-sulfation. Approximately 30% of the D-glucuronyl residues located between two N-sulfated glucosamine units are converted to L-iduronyl units Bos taurus Heparosan-N-sulfate L-iduronate
-
 ?
5.1.3.17 additional information enzyme is involved in the biosynthesis of heparan sulfate in liver Bos taurus ?
-
 ?
5.1.3.17 uronic acid residues
-
 Bos taurus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.1.3.17 ? x * 52000, liver, SDS-PAGE Bos taurus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.1.3.17 620
-
 uronic acid residues
-
 Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.3.17 7 7.5
-
 Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.1.3.17 6.1 7.9 pH 6.1: about 60% of maximal activity, pH 7.9: about 30% of maximal activity Bos taurus