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Literature summary extracted from

  • Campbell, P.; Feingold, D.S.; Jensen, J.W.; Malmström, A.; Roden, L.
    New assay for uronosyl 5-epimerase (1983), Anal. Biochem., 131, 146-152.
    View publication on PubMed

Application

EC Number Application Comment Organism
5.1.3.17 analysis assay procedure for heparosan-N-sulfate-glucuronate 5-epimerase, which is based on the use of a two-phase system for liquid scintillation counting Bos taurus
5.1.3.19 analysis assay procedure for chondroitin-glucuronate 5-epimerase which is based on the use of a two-phase system for liquid scintillation. 3H2O formed during the reaction, is extracted into an organic phase containing fluorine and isoamyl alcohol, while unreacted pol Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.3.17 0.44
-
 uronic acid residues
-
 Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.17 Bos taurus
-
-
-
5.1.3.19 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.1.3.17 liver
-
 Bos taurus
-
5.1.3.19 fibroblast skin Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.17 Heparosan-N-sulfate D-glucuronate
-
 Bos taurus Heparosan-N-sulfate L-iduronate
-
 ?
5.1.3.17 uronic acid residues
-
 Bos taurus ?
-
 ?
5.1.3.19 Chondroitin D-glucuronate
-
 Homo sapiens Dermatan L-iduronate
-
 ?