BRENDA - Enzyme Database

Conformation change of tRNAGlu in the complex with glutamyl-tRNA synthetase is required for specific binding of L-glutamate

Hara-Yokoyama, M.; Yokoyama, S.; Miyazawa, T.; Biochemistry 25, 7031-7036 (1986)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.1.1.17
additional information
-
additional information
as the KCl concentration is raised from 0 to 100 mM, the Km value for L-glutamate in the reaction with E. coli tRNAGlu is remarkably increased wheras the Km value for glutamate with Thermus thermophilus tRNAGlu is slightly increased
Thermus thermophilus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.1.1.17
Thermus thermophilus
-
wild-type and mutant enzymes
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGlu
in absence of tRNAGlu, GluRS binds to D-glutamate as well as L-glutamate
229
Thermus thermophilus
AMP + diphosphate + L-glutamyl-tRNAGlu
-
229
Thermus thermophilus
-
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.1.1.17
additional information
-
additional information
as the KCl concentration is raised from 0 to 100 mM, the Km value for L-glutamate in the reaction with E. coli tRNAGlu is remarkably increased wheras the Km value for glutamate with Thermus thermophilus tRNAGlu is slightly increased
Thermus thermophilus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGlu
in absence of tRNAGlu, GluRS binds to D-glutamate as well as L-glutamate
229
Thermus thermophilus
AMP + diphosphate + L-glutamyl-tRNAGlu
-
229
Thermus thermophilus
-