Literature summary extracted from

Kawamura, T.; Ichihara, N.; Sugiyama, S.; Yokota, H.; Ishimoto, N.; Ito, E.
Biosynthesis of UDP-N-acetyl-D-glucosaminuronic acid and UDP-N-acetyl-D-mannosaminuronic acid in Micrococcus luteus (1985), J. Biochem., 98, 105-116.

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
1.1.1.136	dithiothreitol	and the particulate fraction required for full activity	Micrococcus luteus

General Stability

EC Number	General Stability	Organism
1.1.1.136	ammonium sulfate protects against inactivation during storage and incubation	Micrococcus luteus
1.1.1.136	monovalent cations protect against inactivation during incubation	Micrococcus luteus

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
1.1.1.136	ethanol	10 mM, more than 90% inhibition	Micrococcus luteus
1.1.1.136	N-acetylglucosamine 1-phosphate	-	Micrococcus luteus
1.1.1.136	p-chloromercuribenzoate	0.027 mM, complete inhibition	Micrococcus luteus

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.1.1.136	0.28	-	UDP-N-acetyl-D-glucosamine	-	Micrococcus luteus
1.1.1.136	1.43	-	NAD+	-	Micrococcus luteus

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1.1.1.136	UDP-N-acetyl-D-glucosamine + 2 NAD+ + H2O	Micrococcus luteus	-	UDP-N-acetyl-2-amino-2-deoxy-D-glucuronate + 2 NADH + 2 H+	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.1.1.136	Micrococcus luteus	-	ATCC 4698	-
5.1.3.14	Micrococcus luteus	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.1.1.136	partial	Micrococcus luteus

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.1.1.136	41	-	-	Micrococcus luteus

Storage Stability

EC Number	Storage Stability	Organism
1.1.1.136	4°C, 1 M (NH4)2SO4, stable for more than 3 weeks	Micrococcus luteus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.1.1.136	UDP-N-acetyl-D-glucosamine + 2 NAD+ + H2O	-	Micrococcus luteus	UDP-N-acetyl-2-amino-2-deoxy-D-glucuronate + 2 NADH + 2 H+	-	?

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
1.1.1.136	8.8	-	above, Tris buffer	Micrococcus luteus
1.1.1.136	10	-	above, glycine-NaOH buffer	Micrococcus luteus

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.1.1.136	NAD+	-	Micrococcus luteus

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Release 2023.1 (January 2023)