BRENDA - Enzyme Database

Glutamyl-tRNA synthetase from Escherichia coli

Lapointe, J.; Levasseur, S.; Kern, D.; Methods Enzymol. 113, 42-49 (1985)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.1.1.17
0.0001
-
tRNAGlu
-
Escherichia coli
6.1.1.17
0.1
-
ATP
Glu
Escherichia coli
6.1.1.17
0.2
-
ATP
-
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.1.1.17
Escherichia coli
-
strain MRE-600 and overproducing strain HS7611
-
Purification (Commentary)
EC Number
Commentary
Organism
6.1.1.17
-
Escherichia coli
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.1.1.17
0.79
-
strain HRE-600
Escherichia coli
6.1.1.17
1.31
-
overproducing strain HS7611
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
227
Escherichia coli
AMP + diphosphate + L-glutamyl-tRNAGlu
-
227
Escherichia coli
-
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
6.1.1.17
0.8
-
GlutRNA
-
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.1.1.17
8.6
-
glutamyl-tRNA formation
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.1.1.17
0.0001
-
tRNAGlu
-
Escherichia coli
6.1.1.17
0.1
-
ATP
Glu
Escherichia coli
6.1.1.17
0.2
-
ATP
-
Escherichia coli
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.1.1.17
-
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.1.1.17
0.79
-
strain HRE-600
Escherichia coli
6.1.1.17
1.31
-
overproducing strain HS7611
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
227
Escherichia coli
AMP + diphosphate + L-glutamyl-tRNAGlu
-
227
Escherichia coli
-
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
6.1.1.17
0.8
-
GlutRNA
-
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.1.1.17
8.6
-
glutamyl-tRNA formation
Escherichia coli