Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kawamura, T.; Ishimoto, N.; Ito, E.
    Enzymatic synthesis of uridine diphosphate N-acetyl-D-mannosaminuronic acid (1979), J. Biol. Chem., 254, 8457-8465.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.1.3.14 UDP-N-acetylglucosamine the reverse reaction with UDP-N-acetylmannosamine requires the presence of UDP-N-acetylglucosamine Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.3.14 PCMB
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.3.14 0.63
-
 UDP-N-acetylglucosamine
-
 Escherichia coli
5.1.3.14 1
-
 UDP-N-acetyl-alpha-D-mannosamine in presence of 0.5 mM UDP-N-acetylglucosamine Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.3.14 38000
-
 2 * 38000, SDS-PAGE Escherichia coli
5.1.3.14 76000
-
 gel filtraton Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.3.14 UDP-N-acetyl-D-glucosamine Escherichia coli UDP-N-acetyl-D-glucosamine 2-epimerase and UDP-N-acetyl-D-mannosamine dehydrogenase are responsible for the formation of UDP-N-acetyl-D-mannosaminuronic acid from UDP-N-acetyl-D-glucosamine ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.14 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.3.14
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.1.3.14 7.08
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
5.1.3.14 -18°C, stable for several months Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.14 UDP-N-acetyl-alpha-D-mannosamine
-
 Escherichia coli UDP-N-acetyl-alpha-D-glucosamine
-
 r
5.1.3.14 UDP-N-acetyl-D-glucosamine the reverse reaction with UDP-N-acetylmannosamine requires the presence of UDP-N-acetylglucosamine Escherichia coli UDP-N-acetyl-D-mannosamine
-
 ?
5.1.3.14 UDP-N-acetyl-D-glucosamine UDP-N-acetyl-D-glucosamine 2-epimerase and UDP-N-acetyl-D-mannosamine dehydrogenase are responsible for the formation of UDP-N-acetyl-D-mannosaminuronic acid from UDP-N-acetyl-D-glucosamine Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.1.3.14 dimer 2 * 38000, SDS-PAGE Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.3.14 7 9
-
 Escherichia coli