BRENDA - Enzyme Database

Purification and characterization of glutamyl-tRNA synthetase from an extreme thermophile, Thermus thermophilus HB8

Hara-Yokoyama, M.; Yokoyama, S.; Miyazawa, T.; J. Biochem. 96, 1599-1607 (1984)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.1.1.17
0.0006
-
tRNAGlu
substrate from E. coli
Thermus thermophilus
6.1.1.17
0.00065
-
tRNAGlu
substrate from Thermus thermophilus
Thermus thermophilus
6.1.1.17
0.07
-
L-Glu
-
Thermus thermophilus
6.1.1.17
0.23
-
ATP
-
Thermus thermophilus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.1.1.17
Thermus thermophilus
-
; wild-type and mutant enzymes
-
6.1.1.17
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
6.1.1.17
-
Thermus thermophilus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
226
Thermus thermophilus
AMP + diphosphate + L-glutamyl-tRNAGlu
-
226
Thermus thermophilus
-
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
226
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
AMP + diphosphate + L-glutamyl-tRNAGlu
-
226
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
6.1.1.17
65
-
aminoacylation
Thermus thermophilus
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
6.1.1.17
50
80
about 25% of maximal activity at 50°C and 80°C
Thermus thermophilus
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
6.1.1.17
65
-
9 h, 30% loss of activity
Thermus thermophilus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.1.1.17
8
9
aminoacylation, in presence of 5 mM Mg2+
Thermus thermophilus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.1.1.17
0.0006
-
tRNAGlu
substrate from E. coli
Thermus thermophilus
6.1.1.17
0.00065
-
tRNAGlu
substrate from Thermus thermophilus
Thermus thermophilus
6.1.1.17
0.07
-
L-Glu
-
Thermus thermophilus
6.1.1.17
0.23
-
ATP
-
Thermus thermophilus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.1.1.17
-
Thermus thermophilus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
226
Thermus thermophilus
AMP + diphosphate + L-glutamyl-tRNAGlu
-
226
Thermus thermophilus
-
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
226
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
AMP + diphosphate + L-glutamyl-tRNAGlu
-
226
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
6.1.1.17
65
-
aminoacylation
Thermus thermophilus
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
6.1.1.17
50
80
about 25% of maximal activity at 50°C and 80°C
Thermus thermophilus
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
6.1.1.17
65
-
9 h, 30% loss of activity
Thermus thermophilus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.1.1.17
8
9
aminoacylation, in presence of 5 mM Mg2+
Thermus thermophilus