BRENDA - Enzyme Database

The monomeric glutamyl-tRNA synthetase from Bacillus subtilis 168 and its regulatory factor. Their purification, characterization, and the study of their interaction

Proulx, M.; Duplain, L.; Lacoste, L.; Yaguchi, M.; Lapointe, J.; J. Biol. Chem. 258, 753-759 (1983)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
6.1.1.17
gamma-Globulin
-
Bacillus subtilis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.1.1.17
additional information
-
additional information
-
Bacillus subtilis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.1.1.17
65500
-
1 * 65500, enzyme copurifies with a 46000 MW polypeptide which increases the affinity for glutamate and ATP, and stabilizes it against heat inactivation, SDS-PAGE
Bacillus subtilis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.1.1.17
Bacillus subtilis
-
Trp-
-
6.1.1.17
Bacillus subtilis 168
-
Trp-
-
Purification (Commentary)
EC Number
Commentary
Organism
6.1.1.17
-
Bacillus subtilis
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
6.1.1.17
additional information
-
-
Bacillus subtilis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
221
Bacillus subtilis
AMP + diphosphate + L-glutamyl-tRNAGlu
-
221
Bacillus subtilis
-
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
221
Bacillus subtilis 168
AMP + diphosphate + L-glutamyl-tRNAGlu
-
221
Bacillus subtilis 168
-
Subunits
EC Number
Subunits
Commentary
Organism
6.1.1.17
monomer
1 * 65500, enzyme copurifies with a 46000 MW polypeptide which increases the affinity for glutamate and ATP, and stabilizes it against heat inactivation, SDS-PAGE
Bacillus subtilis
Temperature Stability [░C]
EC Number
Temperature Stability Minimum [░C]
Temperature Stability Maximum [░C]
Commentary
Organism
6.1.1.17
additional information
-
46000 MW polypeptide stabilizes the enzyme against heat inactivation
Bacillus subtilis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
6.1.1.17
gamma-Globulin
-
Bacillus subtilis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.1.1.17
additional information
-
additional information
-
Bacillus subtilis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.1.1.17
65500
-
1 * 65500, enzyme copurifies with a 46000 MW polypeptide which increases the affinity for glutamate and ATP, and stabilizes it against heat inactivation, SDS-PAGE
Bacillus subtilis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.1.1.17
-
Bacillus subtilis
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
6.1.1.17
additional information
-
-
Bacillus subtilis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
221
Bacillus subtilis
AMP + diphosphate + L-glutamyl-tRNAGlu
-
221
Bacillus subtilis
-
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
221
Bacillus subtilis 168
AMP + diphosphate + L-glutamyl-tRNAGlu
-
221
Bacillus subtilis 168
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
6.1.1.17
monomer
1 * 65500, enzyme copurifies with a 46000 MW polypeptide which increases the affinity for glutamate and ATP, and stabilizes it against heat inactivation, SDS-PAGE
Bacillus subtilis
Temperature Stability [░C] (protein specific)
EC Number
Temperature Stability Minimum [░C]
Temperature Stability Maximum [░C]
Commentary
Organism
6.1.1.17
additional information
-
46000 MW polypeptide stabilizes the enzyme against heat inactivation
Bacillus subtilis