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Literature summary extracted from

  • Powers, D.M.; Ginsburg, A.
    Monomeric structure of glutamyl-tRNA synthetase in Escherichia coli (1978), Arch. Biochem. Biophys., 191, 673-679.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.1.1.17 bovine serum albumin optimal activity at 0.25 mg/ml Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.17 0.05
-
 Glu
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.17 Mg2+ required Escherichia coli
6.1.1.17 Mg2+ optimal concentration is 10 mM Escherichia coli
6.1.1.17 Mn2+ can partially replace Mg2+ in activation, maximal efficiency at 5 mM is 71% of Mg2+-activation Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.17 62000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.17 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.17
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.17 ATP + L-glutamate + tRNAGlu
-
 Escherichia coli AMP + diphosphate + L-glutamyl-tRNAGlu
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.17 monomer 1 * 62000, SDS-PAGE Escherichia coli