BRENDA - Enzyme Database

Monomeric structure of glutamyl-tRNA synthetase in Escherichia coli

Powers, D.M.; Ginsburg, A.; Arch. Biochem. Biophys. 191, 673-679 (1978)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
6.1.1.17
bovine serum albumin
optimal activity at 0.25 mg/ml
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.1.1.17
0.05
-
Glu
-
Escherichia coli
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
6.1.1.17
Mg2+
optimal concentration is 10 mM; required
Escherichia coli
6.1.1.17
Mn2+
can partially replace Mg2+ in activation, maximal efficiency at 5 mM is 71% of Mg2+-activation
Escherichia coli
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.1.1.17
62000
-
gel filtration
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.1.1.17
Escherichia coli
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
6.1.1.17
-
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
215
Escherichia coli
AMP + diphosphate + L-glutamyl-tRNAGlu
-
215
Escherichia coli
-
Subunits
EC Number
Subunits
Commentary
Organism
6.1.1.17
monomer
1 * 62000, SDS-PAGE
Escherichia coli
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
6.1.1.17
bovine serum albumin
optimal activity at 0.25 mg/ml
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.1.1.17
0.05
-
Glu
-
Escherichia coli
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
6.1.1.17
Mg2+
optimal concentration is 10 mM; required
Escherichia coli
6.1.1.17
Mn2+
can partially replace Mg2+ in activation, maximal efficiency at 5 mM is 71% of Mg2+-activation
Escherichia coli
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.1.1.17
62000
-
gel filtration
Escherichia coli
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.1.1.17
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
215
Escherichia coli
AMP + diphosphate + L-glutamyl-tRNAGlu
-
215
Escherichia coli
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
6.1.1.17
monomer
1 * 62000, SDS-PAGE
Escherichia coli