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Literature summary extracted from

  • Withe, P.J.; Lejeune, B.; Work, E.
    Assay and properties of diaminopimelate epimerase from Bacillus megaterium (1969), Biochem. J., 113, 589-601.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.1.1.7 additional information pyridoxal phosphate does not stimulate Priestia megaterium
5.1.1.7 thiol compound required for activity Priestia megaterium
5.1.1.7 thiol compound 2,3-dimercaptopropan-1-ol is most effective Priestia megaterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.1.7 6.7
-
LL-diaminoheptanedioate at 37°C Priestia megaterium
5.1.1.7 100
-
meso-diaminoheptanedioate at 37°C Priestia megaterium

Organism

EC Number Organism UniProt Comment Textmining
5.1.1.7 Priestia megaterium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.1.7
-
Priestia megaterium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.1.1.7 2.8
-
-
Priestia megaterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.1.7 LL-2,6-Diaminoheptanedioate r, between 25°C and 45°C at pH 7.0, the equilibrium mixture contains 65% meso-isomer and 35% LL-isomer Priestia megaterium meso-Diaminoheptanedioate
-
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Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.1.7 45
-
the relative rates of epimerization of LL-diaminoheptanedioate at 25°C, 37°C and 45°C are 0.77:1.00:1.15 Priestia megaterium

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.1.1.7 25 45 the relative rates of epimerization of LL-diaminoheptanedioate at 25°C, 37°C and 45°C are 0.77:1.00:1.15 Priestia megaterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.1.7 6.5 8 in both directions Priestia megaterium

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.1.1.7 5.5
-
2°C, overnight, 0.1 mM 2,3-dimercaptopropan-1-ol, 20% loss of activity Priestia megaterium
5.1.1.7 8.5
-
2°C, overnight, 0.1 mM 2,3-dimercaptopropan-1-ol, 70% loss of activity Priestia megaterium