BRENDA - Enzyme Database

Glutamyl transfer ribonucleic acid synthetase of Escherichia coli

Lapointe, J.; Soll, D.; J. Biol. Chem. 247, 4982-4985 (1972)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.1.1.17
0.00012
-
tRNAGlu
56000 MW subunit alone
Escherichia coli
6.1.1.17
0.0003
-
tRNAGlu
56000 MW subunit and 46000 MW subunit
Escherichia coli
6.1.1.17
0.027
-
Glu
56000 MW subunit and 46000 MW subunit
Escherichia coli
6.1.1.17
0.086
-
Glu
56000 MW subunit alone
Escherichia coli
6.1.1.17
0.1
-
ATP
ATP, , 56000 MW subunit and 46000 MW subunit
Escherichia coli
6.1.1.17
0.25
-
ATP
56000 MW subunit alone
Escherichia coli
6.1.1.17
0.32
-
ATP
ATP-diphosphate exchange
Escherichia coli
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.1.1.17
46000
-
1 * 56000 (catalytic subunit) + 1 * 46000 (no detectable enzymatic activity, protective function), SDS-PAGE
Escherichia coli
6.1.1.17
56000
-
1 * 56000 (catalytic subunit) + 1 * 46000 (no detectable enzymatic activity, protective function), SDS-PAGE
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.1.1.17
Escherichia coli
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
213
Escherichia coli
AMP + diphosphate + L-glutamyl-tRNAGlu
-
213
Escherichia coli
-
Subunits
EC Number
Subunits
Commentary
Organism
6.1.1.17
dimer
1 * 56000 (catalytic subunit) + 1 * 46000 (no detectable enzymatic activity, protective function), SDS-PAGE
Escherichia coli
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
6.1.1.17
0.3
-
Glu-tRNA
-
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.1.1.17
8.2
-
glutamyl-tRNA formation
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.1.1.17
0.00012
-
tRNAGlu
56000 MW subunit alone
Escherichia coli
6.1.1.17
0.0003
-
tRNAGlu
56000 MW subunit and 46000 MW subunit
Escherichia coli
6.1.1.17
0.027
-
Glu
56000 MW subunit and 46000 MW subunit
Escherichia coli
6.1.1.17
0.086
-
Glu
56000 MW subunit alone
Escherichia coli
6.1.1.17
0.1
-
ATP
ATP, , 56000 MW subunit and 46000 MW subunit
Escherichia coli
6.1.1.17
0.25
-
ATP
56000 MW subunit alone
Escherichia coli
6.1.1.17
0.32
-
ATP
ATP-diphosphate exchange
Escherichia coli
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.1.1.17
46000
-
1 * 56000 (catalytic subunit) + 1 * 46000 (no detectable enzymatic activity, protective function), SDS-PAGE
Escherichia coli
6.1.1.17
56000
-
1 * 56000 (catalytic subunit) + 1 * 46000 (no detectable enzymatic activity, protective function), SDS-PAGE
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
213
Escherichia coli
AMP + diphosphate + L-glutamyl-tRNAGlu
-
213
Escherichia coli
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
6.1.1.17
dimer
1 * 56000 (catalytic subunit) + 1 * 46000 (no detectable enzymatic activity, protective function), SDS-PAGE
Escherichia coli
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
6.1.1.17
0.3
-
Glu-tRNA
-
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.1.1.17
8.2
-
glutamyl-tRNA formation
Escherichia coli