EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.21.4.1 | NAD+ | - |
Acetoanaerobium sticklandii | |
5.1.1.4 | 2-mercaptoethanol | activates | Acetoanaerobium sticklandii | |
5.1.1.4 | additional information | pyridoxal 5'-phosphate is not involved in racemization | Acetoanaerobium sticklandii | |
5.1.1.4 | SH compounds | activate, e.g. 2-mercaptoethanol, 1,3-dimercaptoethanol | Acetoanaerobium sticklandii |
EC Number | General Stability | Organism |
---|---|---|
1.21.4.1 | withstands ethanol and acetone fractionation at -20°C, does not lose activity as a result of prolonged dialysis | Acetoanaerobium sticklandii |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.21.4.1 | alkaline phosphomonoesterase | preparation from calf intestine, causes extensive inactivation | Acetoanaerobium sticklandii | |
1.21.4.1 | arsenite | - |
Acetoanaerobium sticklandii | |
1.21.4.1 | Co2+ | - |
Acetoanaerobium sticklandii | |
1.21.4.1 | Delta-Aminovalerate | - |
Acetoanaerobium sticklandii | |
1.21.4.1 | Fe2+ | - |
Acetoanaerobium sticklandii | |
1.21.4.1 | human seminal plasma | 50% inactivation by incubation at pH 5.5 for 90 min | Acetoanaerobium sticklandii | |
5.1.1.4 | lipoic acid | weak | Acetoanaerobium sticklandii | |
5.1.1.4 | reduced glutathione | weak | Acetoanaerobium sticklandii | |
5.1.1.4 | thioglycolate | weak | Acetoanaerobium sticklandii |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.21.4.1 | Ca2+ | dialyzed enzyme stimulated by | Acetoanaerobium sticklandii | |
1.21.4.1 | Mg2+ | depend on | Acetoanaerobium sticklandii | |
1.21.4.1 | Mn2+ | dialyzed enzyme stimulated by | Acetoanaerobium sticklandii | |
5.1.1.4 | Mg2+ | 37 mM, 25% inhibition | Acetoanaerobium sticklandii |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
1.21.4.1 | Acetone | withstands fractionation at -20°C, does not lose activity as a result of prolonged dialysis | Acetoanaerobium sticklandii |
1.21.4.1 | Ethanol | withstands fractionation at -20°C, does not lose activity as a result of prolonged dialysis | Acetoanaerobium sticklandii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.21.4.1 | Acetoanaerobium sticklandii | - |
formerly referred to as "an amino acid-fermenting Clostridium, strain HF" | - |
1.21.4.1 | Clostridium sporogenes | - |
- |
- |
5.1.1.4 | Acetoanaerobium sticklandii | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.21.4.1 | - |
Acetoanaerobium sticklandii |
5.1.1.4 | - |
Acetoanaerobium sticklandii |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
5.1.1.4 | L-proline = D-proline | racemization is accompanied by deuterium incorporation from the solvent into the alpha position of Pro, participation of two equivalent hydrogen acceptor sites | Acetoanaerobium sticklandii |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.21.4.1 | 20.4 | - |
- |
Acetoanaerobium sticklandii |
5.1.1.4 | 4.8 | - |
- |
Acetoanaerobium sticklandii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.21.4.1 | D-allohydroxyproline + dihydrolipoate | barely detectable activity | Acetoanaerobium sticklandii | 5-amino-4-hydroxypentanoic acid + lipoate | - |
ir | |
1.21.4.1 | D-proline + 1,3-dimercaptopropanol | - |
Acetoanaerobium sticklandii | 5-aminopentanoic acid + ? | - |
ir | |
1.21.4.1 | D-proline + 1,3-dimercaptopropanol | oxidation of 1,3-dimercaptopropanol cannot be replaced by molecular hydrogen, NADH+ or a number of other reducing agents | Acetoanaerobium sticklandii | 5-aminopentanoic acid + ? | - |
ir | |
1.21.4.1 | D-proline + 6,8-dimercaptooctanoate | - |
Acetoanaerobium sticklandii | 5-aminopentanoic acid + ? | - |
ir | |
1.21.4.1 | D-proline + beta-mercaptoethanol | - |
Acetoanaerobium sticklandii | ? | - |
ir | |
1.21.4.1 | D-proline + dihydrolipoate | - |
Acetoanaerobium sticklandii | delta-aminovaleric acid + lipoate | - |
ir | |
1.21.4.1 | D-proline + dihydrolipoate | - |
Clostridium sporogenes | delta-aminovaleric acid + lipoate | - |
ir | |
1.21.4.1 | D-proline + dithiol (reduced) | - |
Acetoanaerobium sticklandii | 5-aminopentanoic acid + oxidized dithiol | - |
ir | |
1.21.4.1 | D-proline + DL-lipoic acid | - |
Acetoanaerobium sticklandii | 5-aminopentanoic acid + ? | - |
ir | |
5.1.1.4 | L-Pro | - |
Acetoanaerobium sticklandii | D-Pro | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.1.1.4 | 24 | 37 | at 37°C the activity is 35-40% greater than at 24°C | Acetoanaerobium sticklandii |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
5.1.1.4 | 6.6 | 8.1 | pH 5.5: no detectable activity, rapid fall of activity below pH 6.5, activity is relatively stable between pH 6.7 and 8.1 | Acetoanaerobium sticklandii |