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Literature summary extracted from

  • Stadtman, T.C.; Elliott, P.
    Studies on the enzymic reduction of amino acids. II. Purification and properties of a D-proline reductase and a proline racemase from Clostridium sticklandii (1957), J. Biol. Chem., 228, 983-997.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.21.4.1 NAD+
-
Acetoanaerobium sticklandii
5.1.1.4 2-mercaptoethanol activates Acetoanaerobium sticklandii
5.1.1.4 additional information pyridoxal 5'-phosphate is not involved in racemization Acetoanaerobium sticklandii
5.1.1.4 SH compounds activate, e.g. 2-mercaptoethanol, 1,3-dimercaptoethanol Acetoanaerobium sticklandii

General Stability

EC Number General Stability Organism
1.21.4.1 withstands ethanol and acetone fractionation at -20°C, does not lose activity as a result of prolonged dialysis Acetoanaerobium sticklandii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.21.4.1 alkaline phosphomonoesterase preparation from calf intestine, causes extensive inactivation Acetoanaerobium sticklandii
1.21.4.1 arsenite
-
Acetoanaerobium sticklandii
1.21.4.1 Co2+
-
Acetoanaerobium sticklandii
1.21.4.1 Delta-Aminovalerate
-
Acetoanaerobium sticklandii
1.21.4.1 Fe2+
-
Acetoanaerobium sticklandii
1.21.4.1 human seminal plasma 50% inactivation by incubation at pH 5.5 for 90 min Acetoanaerobium sticklandii
5.1.1.4 lipoic acid weak Acetoanaerobium sticklandii
5.1.1.4 reduced glutathione weak Acetoanaerobium sticklandii
5.1.1.4 thioglycolate weak Acetoanaerobium sticklandii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.21.4.1 Ca2+ dialyzed enzyme stimulated by Acetoanaerobium sticklandii
1.21.4.1 Mg2+ depend on Acetoanaerobium sticklandii
1.21.4.1 Mn2+ dialyzed enzyme stimulated by Acetoanaerobium sticklandii
5.1.1.4 Mg2+ 37 mM, 25% inhibition Acetoanaerobium sticklandii

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.21.4.1 Acetone withstands fractionation at -20°C, does not lose activity as a result of prolonged dialysis Acetoanaerobium sticklandii
1.21.4.1 Ethanol withstands fractionation at -20°C, does not lose activity as a result of prolonged dialysis Acetoanaerobium sticklandii

Organism

EC Number Organism UniProt Comment Textmining
1.21.4.1 Acetoanaerobium sticklandii
-
formerly referred to as "an amino acid-fermenting Clostridium, strain HF"
-
1.21.4.1 Clostridium sporogenes
-
-
-
5.1.1.4 Acetoanaerobium sticklandii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.21.4.1
-
Acetoanaerobium sticklandii
5.1.1.4
-
Acetoanaerobium sticklandii

Reaction

EC Number Reaction Comment Organism Reaction ID
5.1.1.4 L-proline = D-proline racemization is accompanied by deuterium incorporation from the solvent into the alpha position of Pro, participation of two equivalent hydrogen acceptor sites Acetoanaerobium sticklandii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.21.4.1 20.4
-
-
Acetoanaerobium sticklandii
5.1.1.4 4.8
-
-
Acetoanaerobium sticklandii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.21.4.1 D-allohydroxyproline + dihydrolipoate barely detectable activity Acetoanaerobium sticklandii 5-amino-4-hydroxypentanoic acid + lipoate
-
ir
1.21.4.1 D-proline + 1,3-dimercaptopropanol
-
Acetoanaerobium sticklandii 5-aminopentanoic acid + ?
-
ir
1.21.4.1 D-proline + 1,3-dimercaptopropanol oxidation of 1,3-dimercaptopropanol cannot be replaced by molecular hydrogen, NADH+ or a number of other reducing agents Acetoanaerobium sticklandii 5-aminopentanoic acid + ?
-
ir
1.21.4.1 D-proline + 6,8-dimercaptooctanoate
-
Acetoanaerobium sticklandii 5-aminopentanoic acid + ?
-
ir
1.21.4.1 D-proline + beta-mercaptoethanol
-
Acetoanaerobium sticklandii ?
-
ir
1.21.4.1 D-proline + dihydrolipoate
-
Acetoanaerobium sticklandii delta-aminovaleric acid + lipoate
-
ir
1.21.4.1 D-proline + dihydrolipoate
-
Clostridium sporogenes delta-aminovaleric acid + lipoate
-
ir
1.21.4.1 D-proline + dithiol (reduced)
-
Acetoanaerobium sticklandii 5-aminopentanoic acid + oxidized dithiol
-
ir
1.21.4.1 D-proline + DL-lipoic acid
-
Acetoanaerobium sticklandii 5-aminopentanoic acid + ?
-
ir
5.1.1.4 L-Pro
-
Acetoanaerobium sticklandii D-Pro
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.1.4 24 37 at 37°C the activity is 35-40% greater than at 24°C Acetoanaerobium sticklandii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.1.1.4 6.6 8.1 pH 5.5: no detectable activity, rapid fall of activity below pH 6.5, activity is relatively stable between pH 6.7 and 8.1 Acetoanaerobium sticklandii