BRENDA - Enzyme Database

Glutamyl transfer ribonucleic acid synthetase of Escherichia coli. II. Interaction with intact glutamyl transfer ribonucleic acid

Lapointe, J.; Sll, D.; J. Biol. Chem. 247, 4975-4981 (1972)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
6.1.1.17
tRNAGlu in presence of Mg2+ protects against heat inactivation, Mg2+ alone is much less effective
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.1.1.17
0.00024
-
tRNA3Glu
-
Escherichia coli
6.1.1.17
0.00042
-
tRNA1Glu
-
Escherichia coli
6.1.1.17
0.00046
-
tRNA2Glu
-
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.1.1.17
Escherichia coli
-
-
-
Reaction
EC Number
Reaction
Commentary
Organism
6.1.1.17
ATP + L-glutamate + tRNAGlu = AMP + diphosphate + L-glutamyl-tRNAGlu
concerted mechanism
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
212
Escherichia coli
AMP + diphosphate + L-glutamyl-tRNAGlu
-
212
Escherichia coli
-
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
6.1.1.17
additional information
-
tRNAGlu in presence of Mg2+ protects against heat inactivation, Mg2+ alone is much less effective
Escherichia coli
General Stability (protein specific)
EC Number
General Stability
Organism
6.1.1.17
tRNAGlu in presence of Mg2+ protects against heat inactivation, Mg2+ alone is much less effective
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.1.1.17
0.00024
-
tRNA3Glu
-
Escherichia coli
6.1.1.17
0.00042
-
tRNA1Glu
-
Escherichia coli
6.1.1.17
0.00046
-
tRNA2Glu
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
212
Escherichia coli
AMP + diphosphate + L-glutamyl-tRNAGlu
-
212
Escherichia coli
-
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
6.1.1.17
additional information
-
tRNAGlu in presence of Mg2+ protects against heat inactivation, Mg2+ alone is much less effective
Escherichia coli