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Literature summary extracted from

  • Baddiley, J; Neuhaus, F.C.
    The enzymic activation of D-alanine (1960), Biochem. J., 75, 579-587.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.13 0.13
-
 diphosphate ATP-diphosphate exchange reaction Lactiplantibacillus plantarum
6.1.1.13 2.5
-
 ATP formation of D-alanine hydroamate Lactiplantibacillus plantarum
6.1.1.13 40
-
 D-Ala formation of D-alanine hydroxamate Lactiplantibacillus plantarum
6.1.1.13 70
-
 D-Ala ATP-diphosphate exchange reaction Lactiplantibacillus plantarum

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.13 Lactiplantibacillus plantarum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.13 partial Lactiplantibacillus plantarum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.13 0.5
-
-
 Lactiplantibacillus plantarum

Storage Stability

EC Number Storage Stability Organism
6.1.1.13 -10°C, ammonium sulfate fraction stable for 6 months Lactiplantibacillus plantarum
6.1.1.13 -20°C, chromatographed fraction loses 25% of activity in 1 week Lactiplantibacillus plantarum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.13 ATP + D-Ala + hydroxylamine D-Ala can be substituted by D-2-amino-n-butyric acid with 14.6% and D-Ser with 7.5% efficiency Lactiplantibacillus plantarum D-Alanine hydroxamate + AMP + diphosphate
-
 ?
6.1.1.13 ATP + diphosphate
-
 Lactiplantibacillus plantarum ATP + diphosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.13 7 8 substrates D-Ala + hydroxylamine + ATP Lactiplantibacillus plantarum
6.1.1.13 7.5 8 ATP-diphosphate exchange reaction Lactiplantibacillus plantarum