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Literature summary extracted from

  • Bode, R.; Schult, I.; Birnbaum, D.
    Purification and some properties of L-threonine dehydratase from Candida maltosa (1986), J. Basic Microbiol., 26, 443-451.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.3.1.19 L-Val activates Candida maltosa
4.3.1.19 phosphate increases activity Candida maltosa
4.3.1.19 phosphate maximal stimulation at 200 mM Candida maltosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.1.19 Hg2+
-
 Candida maltosa
4.3.1.19 Ile 50% inhibition at 0.14 mM Ile, 98% inhibition at 1 mM Ile Candida maltosa
4.3.1.19 PCMB
-
 Candida maltosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.19 3
-
 L-Thr
-
 Candida maltosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.3.1.19 additional information no requirement for divalent cations Candida maltosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.1.19 46000
-
 4 * 46000, SDS-PAGE Candida maltosa
4.3.1.19 190000
-
 gel filtration Candida maltosa

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.19 Candida maltosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.19
-
 Candida maltosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.1.19 additional information
-
-
 Candida maltosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.19 L-homoserine
-
 Candida maltosa 2-oxobutanoate + NH3 + H2O
-
 ?
4.3.1.19 L-serine
-
 Candida maltosa pyruvate + NH3
-
 ?
4.3.1.19 L-threonine
-
 Candida maltosa 2-oxobutanoate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
4.3.1.19 tetramer 4 * 46000, SDS-PAGE Candida maltosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.1.19 8.8
-
-
 Candida maltosa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.3.1.19 8.1 9.6 50% of maximal activity at pH 8.1 and 9.6 Candida maltosa

Cofactor

EC Number Cofactor Comment Organism Structure
4.3.1.19 pyridoxal 5'-phosphate cofactor Candida maltosa
4.3.1.19 pyridoxal 5'-phosphate Km: 0.00028 mM Candida maltosa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.3.1.19 0.14
-
 Ile
-
 Candida maltosa