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Literature summary extracted from
- Purification and properties of D-serine dehydrase from Escherichia coli (1966), J. Biol. Chem., 241, 1233-1238.
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 4.3.1.18 | - |
Escherichia coli |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 4.3.1.18 | resolved completely by dialysis against L-Cys or D-Cys, reactivated by addition of pyridoxal 5'-phosphate | Escherichia coli |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.3.1.18 | O-methylserine | competitive | Escherichia coli |  |
| 4.3.1.18 | Tris | inactivation is prevented by the presence of sufficient K+ or NH4+ and less effectively by Na+ or pyridoxal 5'-phosphate | Escherichia coli | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.3.1.18 | 1.3 | - |
D-Ser | - |
Escherichia coli | |
| 4.3.1.18 | 3.2 | - |
D-Thr | - |
Escherichia coli | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.3.1.18 | 40000 | - |
sucrose density gradient centrifugation | Escherichia coli |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.3.1.18 | Escherichia coli | - |
constitutive mutant | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.3.1.18 | - |
Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.3.1.18 | D-serine | - |
Escherichia coli | pyruvate + NH3 | - |
? | |
| 4.3.1.18 | D-threonine | - |
Escherichia coli | 2-oxobutanoate + NH3 | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.3.1.18 | 7.8 | 8 | - |
Escherichia coli |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.3.1.18 | 6.5 | 9 | pH 6.5: about 30% of maximal activity, pH 9.0: about 60% of maximal activity | Escherichia coli |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.3.1.18 | pyridoxal 5'-phosphate | enzyme linked cofactor | Escherichia coli | |
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Release 2023.1 (January 2023)
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