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Literature summary extracted from

  • Ogawa, H.; Takasugawa, F.; Wakaki, K.; Kishi, H.; Eskandarian, M.R.; Kobayashi, M.; Date, T.; Huh, N.H.; Pitot, H.C.
    Rat liver serine dehydratase. Bacterial expression and two folding domains as revealed by limited proteolysis (1999), J. Biol. Chem., 274, 12855-12860.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.1.17 expression in Escherichia coli Rattus norvegicus

General Stability

EC Number General Stability Organism
4.3.1.17 suszeptible to proteases e.g. trypsin Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.17 50
-
 L-threonine 37°C, pH 8.3, recombinant SDH Rattus norvegicus
4.3.1.17 67
-
 L-serine 37°C, pH 8.3, recombinant SDH Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.1.17 34000
-
 2 * 34000, SDS-PAGE Rattus norvegicus
4.3.1.17 34000
-
 2 * 34000, SDS-PAGE, recombinant SDH Rattus norvegicus
4.3.1.17 66000
-
 gel filtration Rattus norvegicus
4.3.1.17 66000
-
 gel filtration, recombinant SDH Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.1.17 L-serine Rattus norvegicus
-
 pyruvate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.17 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.17 recombinant SDH Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Storage Stability

EC Number Storage Stability Organism
4.3.1.17 -80°C, at least 1 month, no loss of activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.17 L-serine
-
 Rattus norvegicus pyruvate + NH3
-
 ?
4.3.1.17 L-threonine
-
 Rattus norvegicus 2-oxo-butanoate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
4.3.1.17 dimer 2 * 34000, SDS-PAGE Rattus norvegicus
4.3.1.17 dimer 2 * 34000, SDS-PAGE, recombinant SDH Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
4.3.1.17 SDH
-
 Rattus norvegicus
4.3.1.17 Serine dehydratase
-
 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
4.3.1.17 pyridoxal 5'-phosphate
-
 Rattus norvegicus