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Literature summary extracted from

  • Grabowski, R.; Buckel, W.
    Purification and properties of an iron-sulfur containing and pyridoxal-phosphate-independent L-serine dehydratase from Peptostreptococcus asaccharolyticus (1991), Eur. J. Biochem., 199, 89-94.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.1.17 1,10-phenanthroline no effect Peptoniphilus asaccharolyticus
4.3.1.17 EDTA no effect Peptoniphilus asaccharolyticus
4.3.1.17 ethanolamine
-
Peptoniphilus asaccharolyticus
4.3.1.17 Gly
-
Peptoniphilus asaccharolyticus
4.3.1.17 glycylglycine
-
Peptoniphilus asaccharolyticus
4.3.1.17 L-Cys competitive Peptoniphilus asaccharolyticus
4.3.1.17 PCMB
-
Peptoniphilus asaccharolyticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.17 0.8
-
L-Ser
-
Peptoniphilus asaccharolyticus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.3.1.17 Iron the enzyme contains 3.8 mol Fe and 5.6 mol inorganic sulfur per mol of heterodimer, indicating the presence of an [Fe-S]center Peptoniphilus asaccharolyticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.1.17 180000
-
gradient PAGE Peptoniphilus asaccharolyticus
4.3.1.17 200000
-
gel filtration Peptoniphilus asaccharolyticus

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.17 Peptoniphilus asaccharolyticus
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
4.3.1.17 rapid loss of activity upon exposure to air, reactivation by Fe2+ Peptoniphilus asaccharolyticus

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.17
-
Peptoniphilus asaccharolyticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.1.17 additional information
-
-
Peptoniphilus asaccharolyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.17 L-serine specific for L-Ser Peptoniphilus asaccharolyticus pyruvate + NH3
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.1.17 8.4
-
-
Peptoniphilus asaccharolyticus

Cofactor

EC Number Cofactor Comment Organism Structure
4.3.1.17 pyridoxal 5'-phosphate pyridoxal-5'-phosphate-independent enzyme Peptoniphilus asaccharolyticus