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Literature summary extracted from

  • Newman, E.B.; Kapoor, V.
    In vitro studies on L-serine deaminase activity of Escherichia coli K12 (1980), Can. J. Biochem., 58, 1292-1297.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.3.1.17 unstable in all attempts at purification Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.1.17 1,10-phenanthroline
-
 Escherichia coli
4.3.1.17 AlK(SO4)2
-
 Escherichia coli
4.3.1.17 CuCl2 0.7 mM, 50% inhibition Escherichia coli
4.3.1.17 D-Ser
-
 Escherichia coli
4.3.1.17 ethanolamine
-
 Escherichia coli
4.3.1.17 ZnSO4
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.17 420
-
 L-Ser
-
 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.17 Escherichia coli
-
 K12
-

Storage Stability

EC Number Storage Stability Organism
4.3.1.17 -10°C, 40% glycerol, loss of activity within 1 week Escherichia coli
4.3.1.17 0°C, 45% loss of activity after 30 min Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.17 L-serine
-
 Escherichia coli pyruvate + NH3
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.3.1.17
-
-
 30 min, 45% loss of activity Escherichia coli
4.3.1.17 37
-
 complete loss of activity within 30 min. Fe2+, L-Ser D-Ser or ethanolamine decrease the loss of activity at 37°C Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.3.1.17 0.7
-
 CuCl2
-
 Escherichia coli