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Literature summary extracted from

  • Rajaonarivony, J.I.M.; Gershenzon, J.; Croteau, R.
    Characterization and mechanism of (4S)-limonene synthase, a monoterpene cyclase from the glandular trichomes of peppermint (Mentha x piperita) (1992), Arch. Biochem. Biophys., 296, 49-57.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.3.16 Sodium phosphate stimulating at low concentrations Mentha sp.

General Stability

EC Number General Stability Organism
4.2.3.16 unstable in crude secretory cell extract, stable after DEAE-cellulose chromatography Mentha sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.3.16 benzyl bromide
-
 Mentha sp.
4.2.3.16 diethyl dicarbonate
-
 Mentha sp.
4.2.3.16 linalyl sulfonium ion
-
 Mentha sp.
4.2.3.16 N-ethylmaleimide reversible by cysteine treatment Mentha sp.
4.2.3.16 p-hydroxymercuribenzoate pretreatment with geranyl diphosphate and MnCl2 prevents inhibition Mentha sp.
4.2.3.16 Sodium diphosphate Ki: 1 mM Mentha sp.
4.2.3.16 Sodium phosphate Ki: 30 mM Mentha sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.16 0.001
-
 neryl diphosphate
-
 Mentha sp.
4.2.3.16 0.0018
-
 geranyl diphosphate
-
 Mentha sp.
4.2.3.16 0.0019
-
 (3R)-linalyl diphosphate
-
 Mentha sp.
4.2.3.16 0.0023
-
 (3S)-linalyl diphosphate
-
 Mentha sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.16 Mg2+ Mn2+ preferred Mentha sp.
4.2.3.16 Mn2+ required, optimum activity with 2 mM, inhibitory at higher concentrations Mentha sp.
4.2.3.16 additional information Ca2+, Cd2+, Co2+, Cu2+, Fe2+, Ni2+, Zn2+ activated less than 5% Mentha sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.16 geranyl diphosphate Mentha sp.
-
 (-)-(4S)-limonene + diphosphate small amounts of (-)-alpha-pinene, (-)-beta-pinene and myrcene as side products ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.16 Mentha sp.
-
 M. x piperita
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.16 partial Mentha sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.16 leaf
-
 Mentha sp.
-

Storage Stability

EC Number Storage Stability Organism
4.2.3.16 4°C, stable for 1 week without loss of activity after DEAE-cellulose chromatography Mentha sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.16 (3R)-linalyl diphosphate
-
 Mentha sp. ?
-
 ?
4.2.3.16 (3S)-linalyl diphosphate
-
 Mentha sp. ?
-
 ?
4.2.3.16 geranyl diphosphate
-
 Mentha sp. (-)-(4S)-limonene + diphosphate small amounts of (-)-alpha-pinene, (-)-beta-pinene and myrcene as side products ?
4.2.3.16 neryl diphosphate
-
 Mentha sp. ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.16 6.7
-
-
 Mentha sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.3.16 6.1 7.2 half maximum activity at both pH Mentha sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.3.16 1
-
 Sodium diphosphate
-
 Mentha sp.
4.2.3.16 30
-
 Sodium phosphate
-
 Mentha sp.