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Literature summary extracted from

  • Hill, J.
    Purification and properties of streptococcal hyaluronate lyase (1976), Infect. Immun., 14, 726-735.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
4.2.2.1 reducing agents required to stabilize the purified enzyme Streptococcus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.1 0.38
-
 hyaluronate
-
 Streptococcus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.1 50000
-
 x * 50000, SDS-PAGE Streptococcus sp.
4.2.2.1 70000
-
 gel filtration Streptococcus sp.

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.1 Streptococcus sp.
-
 group A type 4
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.2.2.1 side-chain modification glycoprotein Streptococcus sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.1
-
 Streptococcus sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.1 additional information
-
-
 Streptococcus sp.

Storage Stability

EC Number Storage Stability Organism
4.2.2.1 -20°C, stable for 2 years Streptococcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.1 hyaluronate
-
 Streptococcus sp. alpha-4-deoxy-L-threo-hex-4-enopyranosyluronic acid-beta-1,3--N-acetyl-glucosamine
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.1 ? x * 50000, SDS-PAGE Streptococcus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.1 20
-
 room temperature, 3 h, 50% loss of activity Streptococcus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.1 6
-
-
 Streptococcus sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.2.1 6 6.8 pH 6.0: optimum, pH 6.8: 40% of maximal activity Streptococcus sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.2.1 5.5
-
 30 min, irreversible inactivation Streptococcus sp.
4.2.2.1 7
-
 above pH 7.0, 24 h, irreversible loss of activity Streptococcus sp.
4.2.2.1 8.6
-
 10 min, complete loss of activity Streptococcus sp.