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Literature summary extracted from

  • Cheung, K.M.; Spencer, P.; Timko, M.P.; Shoolingin-Jordan, P.M.
    Characterization of a recombinant pea 5-aminolevulinic acid dehydratase and comparative inhibition studies with the Escherichia coli dehydratase (1997), Biochemistry, 36, 1148-1156.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.24 expression in Escherichia coli Pisum sativum

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.24 3-acetyl-4-oxoheptane-1,7-dioic acid formation of a Schiff base complex between the inhibitors and the active site Lys Pisum sativum
4.2.1.24 4-amino-3-oxobutanoate
-
 Pisum sativum
4.2.1.24 Co2+ above 1 mM Pisum sativum
4.2.1.24 Mn2+ above 1 mM Pisum sativum
4.2.1.24 phosphate competitive against Mg2+ Pisum sativum
4.2.1.24 succinylacetone
-
 Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.24 0.8
-
 5-aminolevulinate at pH 7.5 Pisum sativum
4.2.1.24 1
-
 5-aminolevulinate at pH 9 Pisum sativum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.24 Co2+ activates Pisum sativum
4.2.1.24 Co2+ about 50% reactivation of the demetalled protein Pisum sativum
4.2.1.24 Mg2+ required Pisum sativum
4.2.1.24 Mg2+ activates Pisum sativum
4.2.1.24 Mg2+ 2 Mg2+ binding sites per subunit Pisum sativum
4.2.1.24 Mn2+ activates Pisum sativum
4.2.1.24 Mn2+ can reactivate the demetallated protein Pisum sativum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.24 43000
-
 8 * 43000, SDS-PAGE Pisum sativum
4.2.1.24 309000
-
 gel filtration Pisum sativum

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.24 Pisum sativum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.24
-
 Pisum sativum

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.1.24 2 5-aminolevulinate = porphobilinogen + 2 H2O substrate is covalently bound through a Schiff base Pisum sativum
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.24 4.33
-
-
 Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.24 5-aminolevulinate + 5-aminolevulinate
-
 Pisum sativum porphobilinogen + 2 H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.24 octamer 8 * 43000, SDS-PAGE Pisum sativum