BRENDA - Enzyme Database show

Characterization of a recombinant pea 5-aminolevulinic acid dehydratase and comparative inhibition studies with the Escherichia coli dehydratase

Cheung, K.M.; Spencer, P.; Timko, M.P.; Shoolingin-Jordan, P.M.; Biochemistry 36, 1148-1156 (1997)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.24
expression in Escherichia coli
Pisum sativum
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.24
3-acetyl-4-oxoheptane-1,7-dioic acid
formation of a Schiff base complex between the inhibitors and the active site Lys
Pisum sativum
4.2.1.24
4-amino-3-oxobutanoate
-
Pisum sativum
4.2.1.24
Co2+
above 1 mM
Pisum sativum
4.2.1.24
Mn2+
above 1 mM
Pisum sativum
4.2.1.24
phosphate
competitive against Mg2+
Pisum sativum
4.2.1.24
succinylacetone
-
Pisum sativum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.24
0.8
-
5-aminolevulinate
at pH 7.5
Pisum sativum
4.2.1.24
1
-
5-aminolevulinate
at pH 9
Pisum sativum
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.24
Co2+
about 50% reactivation of the demetalled protein; activates
Pisum sativum
4.2.1.24
Mg2+
2 Mg2+ binding sites per subunit; activates; required
Pisum sativum
4.2.1.24
Mn2+
activates; can reactivate the demetallated protein
Pisum sativum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.24
43000
-
8 * 43000, SDS-PAGE
Pisum sativum
4.2.1.24
309000
-
gel filtration
Pisum sativum
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.24
Pisum sativum
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.24
-
Pisum sativum
Reaction
EC Number
Reaction
Commentary
Organism
4.2.1.24
2 5-aminolevulinate = porphobilinogen + 2 H2O
substrate is covalently bound through a Schiff base
Pisum sativum
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.24
4.33
-
-
Pisum sativum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.24
5-aminolevulinate + 5-aminolevulinate
-
210729
Pisum sativum
porphobilinogen + 2 H2O
-
210729
Pisum sativum
?
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.24
octamer
8 * 43000, SDS-PAGE
Pisum sativum
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.24
expression in Escherichia coli
Pisum sativum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.24
3-acetyl-4-oxoheptane-1,7-dioic acid
formation of a Schiff base complex between the inhibitors and the active site Lys
Pisum sativum
4.2.1.24
4-amino-3-oxobutanoate
-
Pisum sativum
4.2.1.24
Co2+
above 1 mM
Pisum sativum
4.2.1.24
Mn2+
above 1 mM
Pisum sativum
4.2.1.24
phosphate
competitive against Mg2+
Pisum sativum
4.2.1.24
succinylacetone
-
Pisum sativum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.24
0.8
-
5-aminolevulinate
at pH 7.5
Pisum sativum
4.2.1.24
1
-
5-aminolevulinate
at pH 9
Pisum sativum
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.24
Co2+
about 50% reactivation of the demetalled protein; activates
Pisum sativum
4.2.1.24
Mg2+
2 Mg2+ binding sites per subunit; activates; required
Pisum sativum
4.2.1.24
Mn2+
activates; can reactivate the demetallated protein
Pisum sativum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.24
43000
-
8 * 43000, SDS-PAGE
Pisum sativum
4.2.1.24
309000
-
gel filtration
Pisum sativum
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.24
-
Pisum sativum
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.24
4.33
-
-
Pisum sativum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.24
5-aminolevulinate + 5-aminolevulinate
-
210729
Pisum sativum
porphobilinogen + 2 H2O
-
210729
Pisum sativum
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.24
octamer
8 * 43000, SDS-PAGE
Pisum sativum