BRENDA - Enzyme Database show

Purification of delta-aminolevulinate dehydratase from genetically engineered yeast

Borralho, L.M.; Ortiz, C.H.D.; Panek, A.D.; Mattoon, J.R.; Yeast 6, 319-330 (1990)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.24
Cu2+
-
Saccharomyces cerevisiae
4.2.1.24
Pb2+
-
Saccharomyces cerevisiae
4.2.1.24
Zn2+
activity is progressively increased with increasing Zn2+ concentrations up to 0.1 mM, inhibition at high concentrations
Saccharomyces cerevisiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.24
0.359
-
5-aminolevulinate
-
Saccharomyces cerevisiae
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.24
Zn2+
activity is progressively increased with increasing Zn2+ concentrations up to 0.1 mM, inhibition at high concentrations
Saccharomyces cerevisiae
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.24
37000
-
8 * 37000, SDS-PAGE
Saccharomyces cerevisiae
4.2.1.24
275000
-
glycerol density gradient centrifugation
Saccharomyces cerevisiae
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.24
Saccharomyces cerevisiae
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.24
-
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.24
0.27
-
-
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.24
5-aminolevulinate + 5-aminolevulinate
-
210725
Saccharomyces cerevisiae
porphobilinogen + 2 H2O
-
210725
Saccharomyces cerevisiae
?
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.24
octamer
8 * 37000, SDS-PAGE
Saccharomyces cerevisiae
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.2.1.24
45
-
-
Saccharomyces cerevisiae
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.2.1.24
50
-
15 min, 63% loss of activity
Saccharomyces cerevisiae
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.24
9.8
-
-
Saccharomyces cerevisiae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.24
Cu2+
-
Saccharomyces cerevisiae
4.2.1.24
Pb2+
-
Saccharomyces cerevisiae
4.2.1.24
Zn2+
activity is progressively increased with increasing Zn2+ concentrations up to 0.1 mM, inhibition at high concentrations
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.24
0.359
-
5-aminolevulinate
-
Saccharomyces cerevisiae
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.24
Zn2+
activity is progressively increased with increasing Zn2+ concentrations up to 0.1 mM, inhibition at high concentrations
Saccharomyces cerevisiae
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.24
37000
-
8 * 37000, SDS-PAGE
Saccharomyces cerevisiae
4.2.1.24
275000
-
glycerol density gradient centrifugation
Saccharomyces cerevisiae
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.24
-
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.24
0.27
-
-
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.24
5-aminolevulinate + 5-aminolevulinate
-
210725
Saccharomyces cerevisiae
porphobilinogen + 2 H2O
-
210725
Saccharomyces cerevisiae
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.24
octamer
8 * 37000, SDS-PAGE
Saccharomyces cerevisiae
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.2.1.24
45
-
-
Saccharomyces cerevisiae
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.2.1.24
50
-
15 min, 63% loss of activity
Saccharomyces cerevisiae
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.24
9.8
-
-
Saccharomyces cerevisiae