BRENDA - Enzyme Database show

Purification, metal cofactor, N-terminal sequence and subunit composition of a 5-aminolevulinic acid dehydratase from the unicellular green alga Scenedesmus obliquus, mutant C-2A'

Stolz, M.; Dörnemann, D.; Eur. J. Biochem. 236, 600-608 (1996)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
4.2.1.24
EGTA prevents proteolysis
Tetradesmus obliquus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.24
0.26
-
5-aminolevulinate
-
Tetradesmus obliquus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.24
Ca2+
about 80% reactivation of the demetalled protein
Tetradesmus obliquus
4.2.1.24
Co2+
about 70% reactivation of the demetalled protein
Tetradesmus obliquus
4.2.1.24
Fe2+
about 70% reactivation of the demetalled protein
Tetradesmus obliquus
4.2.1.24
Mg2+
metal cofactor
Tetradesmus obliquus
4.2.1.24
Mn2+
can reactivate the demetallated protein
Tetradesmus obliquus
4.2.1.24
Ni(2+)
about 70% reactivation of the demetalled protein
Tetradesmus obliquus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.24
42000
-
6 * 42000, SDS-PAGE
Tetradesmus obliquus
4.2.1.24
282000
-
gel filtration
Tetradesmus obliquus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.24
Tetradesmus obliquus
-
mutant strain C-2A'
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.24
-
Tetradesmus obliquus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.24
additional information
-
-
Tetradesmus obliquus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.24
5-aminolevulinate + 5-aminolevulinate
-
210720
Tetradesmus obliquus
porphobilinogen + 2 H2O
-
210720
Tetradesmus obliquus
?
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.24
hexamer
6 * 42000, SDS-PAGE
Tetradesmus obliquus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.24
8.3
-
-
Tetradesmus obliquus
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
4.2.1.24
9
-
highest stability at
Tetradesmus obliquus
General Stability (protein specific)
EC Number
General Stability
Organism
4.2.1.24
EGTA prevents proteolysis
Tetradesmus obliquus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.24
0.26
-
5-aminolevulinate
-
Tetradesmus obliquus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.24
Ca2+
about 80% reactivation of the demetalled protein
Tetradesmus obliquus
4.2.1.24
Co2+
about 70% reactivation of the demetalled protein
Tetradesmus obliquus
4.2.1.24
Fe2+
about 70% reactivation of the demetalled protein
Tetradesmus obliquus
4.2.1.24
Mg2+
metal cofactor
Tetradesmus obliquus
4.2.1.24
Mn2+
can reactivate the demetallated protein
Tetradesmus obliquus
4.2.1.24
Ni(2+)
about 70% reactivation of the demetalled protein
Tetradesmus obliquus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.24
42000
-
6 * 42000, SDS-PAGE
Tetradesmus obliquus
4.2.1.24
282000
-
gel filtration
Tetradesmus obliquus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.24
-
Tetradesmus obliquus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.24
additional information
-
-
Tetradesmus obliquus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.24
5-aminolevulinate + 5-aminolevulinate
-
210720
Tetradesmus obliquus
porphobilinogen + 2 H2O
-
210720
Tetradesmus obliquus
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.24
hexamer
6 * 42000, SDS-PAGE
Tetradesmus obliquus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.24
8.3
-
-
Tetradesmus obliquus
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
4.2.1.24
9
-
highest stability at
Tetradesmus obliquus