BRENDA - Enzyme Database show

delta-Aminolevulinic acid dehydratase of Rhodopseudomonas capsulata

Nandi, D.L.; Shemin, D.; Arch. Biochem. Biophys. 158, 305-311 (1973)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
4.2.1.24
2-mercaptoethanol
required at very low concentrations of substrate
Rhodobacter capsulatus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.24
EDTA
no inhibition
Rhodobacter capsulatus
4.2.1.24
iodoacetamide
-
Rhodobacter capsulatus
4.2.1.24
additional information
insensitive to inhibition by hemin and protoporphyrin
Rhodobacter capsulatus
4.2.1.24
NaCN
in presence of the substrate
Rhodobacter capsulatus
4.2.1.24
NEM
-
Rhodobacter capsulatus
4.2.1.24
PCMB
-
Rhodobacter capsulatus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.24
0.7
-
5-aminolevulinate
in presence of 2-mercaptoethanol
Rhodobacter capsulatus
4.2.1.24
2
-
5-aminolevulinate
in absence of 2-mercaptoethanol
Rhodobacter capsulatus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.24
additional information
does not require metallic cations for activation
Rhodobacter capsulatus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.24
260000
-
sucrose density gradient centrifugation
Rhodobacter capsulatus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.24
Rhodobacter capsulatus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.24
-
Rhodobacter capsulatus
Reaction
EC Number
Reaction
Commentary
Organism
4.2.1.24
2 5-aminolevulinate = porphobilinogen + 2 H2O
substrate is covalently bound through a Schiff base
Rhodobacter capsulatus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.24
1.62
-
-
Rhodobacter capsulatus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.24
5-aminolevulinate + 5-aminolevulinate
-
210707
Rhodobacter capsulatus
porphobilinogen + 2 H2O
-
210707
Rhodobacter capsulatus
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.24
8
8.6
in citrate buffer, Tris-maleate buffer, imidazole buffer and Tris buffer
Rhodobacter capsulatus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
4.2.1.24
2-mercaptoethanol
required at very low concentrations of substrate
Rhodobacter capsulatus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.24
EDTA
no inhibition
Rhodobacter capsulatus
4.2.1.24
iodoacetamide
-
Rhodobacter capsulatus
4.2.1.24
additional information
insensitive to inhibition by hemin and protoporphyrin
Rhodobacter capsulatus
4.2.1.24
NaCN
in presence of the substrate
Rhodobacter capsulatus
4.2.1.24
NEM
-
Rhodobacter capsulatus
4.2.1.24
PCMB
-
Rhodobacter capsulatus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.24
0.7
-
5-aminolevulinate
in presence of 2-mercaptoethanol
Rhodobacter capsulatus
4.2.1.24
2
-
5-aminolevulinate
in absence of 2-mercaptoethanol
Rhodobacter capsulatus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.24
additional information
does not require metallic cations for activation
Rhodobacter capsulatus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.24
260000
-
sucrose density gradient centrifugation
Rhodobacter capsulatus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.24
-
Rhodobacter capsulatus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.24
1.62
-
-
Rhodobacter capsulatus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.24
5-aminolevulinate + 5-aminolevulinate
-
210707
Rhodobacter capsulatus
porphobilinogen + 2 H2O
-
210707
Rhodobacter capsulatus
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.24
8
8.6
in citrate buffer, Tris-maleate buffer, imidazole buffer and Tris buffer
Rhodobacter capsulatus