BRENDA - Enzyme Database show

Purification and properties of delta-aminolevulinate dehydrase from human erythrocytes

Anderson, P.M.; Desnick, R.J.; J. Biol. Chem. 254, 6924-6930 (1979)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.24
Fe2+
noncompetitive
Homo sapiens
4.2.1.24
pyridoxal 5'-phosphate
competitive
Homo sapiens
4.2.1.24
Zn2+
activates at 0.1-0.02 mM, inhibits at 1.0 mM
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.24
0.27
-
5-aminolevulinate
-
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.24
Zn2+
activates at 0.1-0.02 mM, inhibits at 1.0 mM
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.24
31000
-
8 * 31000, SDS-PAGE
Homo sapiens
4.2.1.24
252000
-
gel filtration
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.24
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.24
-
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.24
erythrocyte
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.24
0.31
-
-
Homo sapiens
Storage Stability
EC Number
Storage Stability
Organism
4.2.1.24
-20°C, protein concentration above 2 mg/ml, 20 mM phosphate, 5 mM dithiothreitol, pH 6.8, stable for 6 months
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.24
5-aminolevulinate + 5-aminolevulinate
-
210699
Homo sapiens
porphobilinogen + 2 H2O
-
210699
Homo sapiens
?
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.24
octamer
8 * 31000, SDS-PAGE
Homo sapiens
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.2.1.24
50
-
200 min, 50% inactivation
Homo sapiens
4.2.1.24
55
-
90 min, 50% inactivation
Homo sapiens
4.2.1.24
60
-
30 min, 50% inactivation
Homo sapiens
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.2.1.24
additional information
-
additional information
-
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.24
6.3
6.7
-
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.24
Fe2+
noncompetitive
Homo sapiens
4.2.1.24
pyridoxal 5'-phosphate
competitive
Homo sapiens
4.2.1.24
Zn2+
activates at 0.1-0.02 mM, inhibits at 1.0 mM
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.24
0.27
-
5-aminolevulinate
-
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.24
Zn2+
activates at 0.1-0.02 mM, inhibits at 1.0 mM
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.24
31000
-
8 * 31000, SDS-PAGE
Homo sapiens
4.2.1.24
252000
-
gel filtration
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.24
-
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.24
erythrocyte
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.24
0.31
-
-
Homo sapiens
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
4.2.1.24
-20°C, protein concentration above 2 mg/ml, 20 mM phosphate, 5 mM dithiothreitol, pH 6.8, stable for 6 months
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.24
5-aminolevulinate + 5-aminolevulinate
-
210699
Homo sapiens
porphobilinogen + 2 H2O
-
210699
Homo sapiens
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.24
octamer
8 * 31000, SDS-PAGE
Homo sapiens
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.2.1.24
50
-
200 min, 50% inactivation
Homo sapiens
4.2.1.24
55
-
90 min, 50% inactivation
Homo sapiens
4.2.1.24
60
-
30 min, 50% inactivation
Homo sapiens
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.2.1.24
additional information
-
additional information
-
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.24
6.3
6.7
-
Homo sapiens