BRENDA - Enzyme Database show

Purification and properties of 5-aminolevulinate dehydratase from human erythrocytes

Gibbs, P.N.B.; Chaudhry, A.G.; Jordan, P.M.; Biochem. J. 230, 25-34 (1985)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.24
5,5'-dithiobis(2-nitrobenzoic acid)
-
Homo sapiens
4.2.1.24
EDTA
-
Homo sapiens
4.2.1.24
Pb2+
noncompetitive
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.24
0.287
-
5-aminolevulinate
-
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.24
Zn2+
optimal activation by 0.1-0.3 mM ZnCl2; required
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.24
35000
-
8 * 35000, SDS-PAGE
Homo sapiens
4.2.1.24
285000
-
gel filtration
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.24
Homo sapiens
-
-
-
Oxidation Stability
EC Number
Oxidation Stability
Organism
4.2.1.24
sensitive to oxygen, t1/2: 135 min, the oxygen-inactivated enzyme is restored to full activity by incubation with thiols
Homo sapiens
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.24
-
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.24
erythrocyte
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.24
0.4
-
-
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.24
5-aminolevulinate + 5-aminolevulinate
-
210688
Homo sapiens
porphobilinogen + 2 H2O
-
210688
Homo sapiens
?
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.24
octamer
8 * 35000, SDS-PAGE
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.24
6.8
-
-
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.24
5,5'-dithiobis(2-nitrobenzoic acid)
-
Homo sapiens
4.2.1.24
EDTA
-
Homo sapiens
4.2.1.24
Pb2+
noncompetitive
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.24
0.287
-
5-aminolevulinate
-
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.24
Zn2+
optimal activation by 0.1-0.3 mM ZnCl2; required
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.24
35000
-
8 * 35000, SDS-PAGE
Homo sapiens
4.2.1.24
285000
-
gel filtration
Homo sapiens
Oxidation Stability (protein specific)
EC Number
Oxidation Stability
Organism
4.2.1.24
sensitive to oxygen, t1/2: 135 min, the oxygen-inactivated enzyme is restored to full activity by incubation with thiols
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.24
-
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.24
erythrocyte
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.24
0.4
-
-
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.24
5-aminolevulinate + 5-aminolevulinate
-
210688
Homo sapiens
porphobilinogen + 2 H2O
-
210688
Homo sapiens
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.24
octamer
8 * 35000, SDS-PAGE
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.24
6.8
-
-
Homo sapiens