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Literature summary extracted from
- Purification and properties of the inducible enzyme cyanase (1980), Biochemistry, 19, 2882-2888.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.104 | 0.6 | - |
Cyanate | - |
Escherichia coli |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.104 | 14661 | - |
x * 14661, minimum molecular weight calculated from amino acid composition | Escherichia coli |
| 4.2.1.104 | 15200 | - |
x * 15200, SDS-PAGE | Escherichia coli |
| 4.2.1.104 | 150000 | - |
E. coli, sucrose density gradient centrifugation, gel filtration | Escherichia coli |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.104 | Escherichia coli | - |
B | - |
| 4.2.1.104 | Escherichia coli B / ATCC 11303 | - |
B | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.104 | B | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.104 | Cyanate + bicarbonate | - |
Escherichia coli | CO2 + carbamate | initial product is carbamate or a related, unstable compound and/or carbamate precursor, which subsequently decomposes to ammonia and bicarbonate | ? | |
| 4.2.1.104 | Cyanate + bicarbonate | - |
Escherichia coli B / ATCC 11303 | CO2 + carbamate | initial product is carbamate or a related, unstable compound and/or carbamate precursor, which subsequently decomposes to ammonia and bicarbonate | ? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.1.104 | oligomer | x * 15200, SDS-PAGE | Escherichia coli |
| 4.2.1.104 | oligomer | x * 14661, minimum molecular weight calculated from amino acid composition | Escherichia coli |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.104 | 37 | - |
assay at | Escherichia coli |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.104 | 7.4 | - |
- |
Escherichia coli |
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Release 2023.1 (January 2023)
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