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Literature summary extracted from

  • Middleton, K.; Carbon, J.
    KAR3-encoded kinesin is a minus-end-directed motor that functions with centromere binding proteins (CBF3) on an in vitro yeast kinetochore (1994), Proc. Natl. Acad. Sci. USA, 91, 7212-7216.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.6.1.4 KAR3 gene cloned, Kar3p overexpressed in Escherichia coli Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.6.1.4 80000
-
-
 Drosophila melanogaster
5.6.1.4 80000
-
 Kar3p, SDS-PAGE Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.6.1.4 ATP + H2O + a kinesin associated with a microtubule at position n Drosophila melanogaster
-
 ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
 ?
5.6.1.4 ATP + H2O + a kinesin associated with a microtubule at position n Saccharomyces cerevisiae
-
 ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.6.1.4 Drosophila melanogaster
-
-
-
5.6.1.4 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.6.1.4
-
 Saccharomyces cerevisiae

Reaction

EC Number Reaction Comment Organism Reaction ID
5.6.1.4 ATP + H2O + a kinesin associated with a microtubule at position n = ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end) structurally almost identical to EC3.6.4.3 (microtubule-severing ATPase) but the movement it catalyses is towards the minus end of microtubules Drosophila melanogaster
a
5.6.1.4 ATP + H2O + a kinesin associated with a microtubule at position n = ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end) structurally almost identical to EC3.6.4.3 (microtubule-severing ATPase) but the movement it catalyses is towards the minus end of microtubules Saccharomyces cerevisiae
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.1.4 ATP + H2O + a kinesin associated with a microtubule at position n
-
 Drosophila melanogaster ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
 ?
5.6.1.4 ATP + H2O + a kinesin associated with a microtubule at position n
-
 Saccharomyces cerevisiae ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.6.1.4 Kar3
-
 Saccharomyces cerevisiae
5.6.1.4 KAR3-encoded kinesin
-
 Saccharomyces cerevisiae