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Literature summary extracted from

  • Obis, D.; Guillot, A.; Gripon, J.C.; Renault, P.; Bolotin, A.; Mistou, M.Y.
    Genetic and biochemical characterization of a high-affinity betaine uptake system (BusA) in Lactococcus lactis reveals a new functional organization within bacterial ABC transporters (1999), J. Bacteriol., 181, 6238-6246.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.6.2.9
-
 Lactococcus lactis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.6.2.9 0.0017
-
 betaine
-
 Lactococcus lactis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.6.2.9 45679
-
 x * 45679, BusAA + x * 61966, BusAB + x * ?, BusAC, calculation from nucleotide sequence Lactococcus lactis
7.6.2.9 61966
-
 x * 45679, BusAA + x * 61966, BusAB + x * ?, BusAC, calculation from nucleotide sequence Lactococcus lactis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.6.2.9 ATP + H2O + glycine betaine/out Lactococcus lactis enzyme is involved in cytoplasmic accumulation of exogenous betaine which has osmoprotective properties, transcription of busA is strongly regulated by the external osmolarity of the medium ADP + phosphate + glycine betaine/in
-
 ?
7.6.2.9 ATP + H2O + glycine betaine/out Lactococcus lactis OpuA enzyme is involved in cytoplasmic accumulation of exogenous betaine which has osmoprotective properties, transcription of busA is strongly regulated by the external osmolarity of the medium ADP + phosphate + glycine betaine/in
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.6.2.9 Lactococcus lactis
-
 OpuA
-
7.6.2.9 Lactococcus lactis OpuA
-
 OpuA
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.6.2.9 ATP + H2O + betaine/out
-
 Lactococcus lactis ADP + phosphate + betaine/in
-
 ?
7.6.2.9 ATP + H2O + betaine/out
-
 Lactococcus lactis OpuA ADP + phosphate + betaine/in
-
 ?
7.6.2.9 ATP + H2O + glycine betaine/out
-
 Lactococcus lactis ADP + phosphate + glycine betaine/in
-
 ?
7.6.2.9 ATP + H2O + glycine betaine/out enzyme is involved in cytoplasmic accumulation of exogenous betaine which has osmoprotective properties, transcription of busA is strongly regulated by the external osmolarity of the medium Lactococcus lactis ADP + phosphate + glycine betaine/in
-
 ?
7.6.2.9 ATP + H2O + glycine betaine/out
-
 Lactococcus lactis OpuA ADP + phosphate + glycine betaine/in
-
 ?
7.6.2.9 ATP + H2O + glycine betaine/out enzyme is involved in cytoplasmic accumulation of exogenous betaine which has osmoprotective properties, transcription of busA is strongly regulated by the external osmolarity of the medium Lactococcus lactis OpuA ADP + phosphate + glycine betaine/in
-
 ?

Subunits

EC Number Subunits Comment Organism
7.6.2.9 ? x * 45679, BusAA + x * 61966, BusAB + x * ?, BusAC, calculation from nucleotide sequence Lactococcus lactis