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Literature summary extracted from

  • Reidlinger, J.; Muller, V.
    Purification of ATP synthase from Acetobacterium woodii and identification as a Na+-translocating F1F0-type enzyme (1994), Eur. J. Biochem., 223, 275-283.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
7.2.2.1 sulfite stimulates activity Acetobacterium woodii
7.2.2.3 SO32-
-
 Acetobacterium woodii

Application

EC Number Application Comment Organism
7.2.2.3 medicine ATP hydrolysis Acetobacterium woodii

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.2.2.1 5-(N,N-hexamethylene)-amiloride
-
 Acetobacterium woodii
7.2.2.1 5-(N-ethyl-N-isopropyl)-amiloride
-
 Acetobacterium woodii
7.2.2.1 ADP
-
 Acetobacterium woodii
7.2.2.1 azide
-
 Acetobacterium woodii
7.2.2.1 Ca2+
-
 Acetobacterium woodii
7.2.2.1 Cr2+
-
 Acetobacterium woodii
7.2.2.1 Cu2+
-
 Acetobacterium woodii
7.2.2.1 N,N'-dicyclohexylcarbodiimide reacts with the 4800 Da subunit Acetobacterium woodii
7.2.2.1 N-10-benzylamiloride
-
 Acetobacterium woodii
7.2.2.1 Ni2+
-
 Acetobacterium woodii
7.2.2.1 NO3-
-
 Acetobacterium woodii
7.2.2.3 5-(N,N'-hexamethylene)-amiloride
-
 Acetobacterium woodii
7.2.2.3 5-(N-ethyl-N-isopropyl)-amiloride
-
 Acetobacterium woodii
7.2.2.3 Dicyclohexylcarbodiimide
-
 Acetobacterium woodii
7.2.2.3 N-10-benzyl-amiloride
-
 Acetobacterium woodii
7.2.2.3 nitrate
-
 Acetobacterium woodii
7.2.2.3 Sodium azide
-
 Acetobacterium woodii
7.2.2.3 vanadate
-
 Acetobacterium woodii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.2.2.1 membrane
-
 Acetobacterium woodii 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.2.2.1 Mg2+ activity is dependent on MgATP, at pH 8.5 optimal Mg2+ concentration is 2 mM Acetobacterium woodii
7.2.2.1 Na+ the enzyme has Na+-translocating activity Acetobacterium woodii
7.2.2.1 Na+ strictly dependent on Na+, Km: 0.4 mM Acetobacterium woodii
7.2.2.3 Na+
-
 Acetobacterium woodii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.2.2.1 15000
-
 x * 4800 + x * 15000 + x * 19000 + x * 35000 + x * 52000 + x * 57000, at least 6 subunits, SDS-PAGE Acetobacterium woodii
7.2.2.1 19000
-
 x * 4800 + x * 15000 + x * 19000 + x * 35000 + x * 52000 + x * 57000, at least 6 subunits, SDS-PAGE Acetobacterium woodii
7.2.2.1 35000
-
 x * 4800 + x * 15000 + x * 19000 + x * 35000 + x * 52000 + x * 57000, at least 6 subunits, SDS-PAGE Acetobacterium woodii
7.2.2.1 52000
-
 x * 4800 + x * 15000 + x * 19000 + x * 35000 + x * 52000 + x * 57000, at least 6 subunits, SDS-PAGE Acetobacterium woodii
7.2.2.1 57000
-
 x * 4800 + x * 15000 + x * 19000 + x * 35000 + x * 52000 + x * 57000, at least 6 subunits, SDS-PAGE Acetobacterium woodii
7.2.2.3 15000
-
 1 * 57000 + 1 * 52000 + 1 * 35000 + 1 * 19000 + 1 * 15000 + 1 * 4800, SDS-PAGE Acetobacterium woodii
7.2.2.3 19000
-
 1 * 57000 + 1 * 52000 + 1 * 35000 + 1 * 19000 + 1 * 15000 + 1 * 4800, SDS-PAGE Acetobacterium woodii
7.2.2.3 35000
-
 1 * 57000 + 1 * 52000 + 1 * 35000 + 1 * 19000 + 1 * 15000 + 1 * 4800, SDS-PAGE Acetobacterium woodii
7.2.2.3 52000
-
 1 * 57000 + 1 * 52000 + 1 * 35000 + 1 * 19000 + 1 * 15000 + 1 * 4800, SDS-PAGE Acetobacterium woodii
7.2.2.3 57000
-
 1 * 57000 + 1 * 52000 + 1 * 35000 + 1 * 19000 + 1 * 15000 + 1 * 4800, SDS-PAGE Acetobacterium woodii

Organism

EC Number Organism UniProt Comment Textmining
7.2.2.1 Acetobacterium woodii
-
-
-
7.2.2.3 Acetobacterium woodii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.2.2.1
-
 Acetobacterium woodii
7.2.2.3
-
 Acetobacterium woodii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.2.2.1 additional information
-
-
 Acetobacterium woodii
7.2.2.3 16
-
-
 Acetobacterium woodii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.2.2.1 ATP + H2O
-
 Acetobacterium woodii ADP + phosphate
-
 ?
7.2.2.1 CTP + H2O at 9% of the activity with ATP Acetobacterium woodii CDP + H2O
-
 ?
7.2.2.1 GTP + H2O hydrolyzed at 31% of the activity with ATP Acetobacterium woodii GDP + phosphate
-
 ?
7.2.2.1 ITP + H2O hydrolyzed at 39% of the activity with ATP Acetobacterium woodii ?
-
 ?
7.2.2.1 UTP + H2O at 4% of the activity with ATP Acetobacterium woodii UDP + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
7.2.2.1 ? x * 4800 + x * 15000 + x * 19000 + x * 35000 + x * 52000 + x * 57000, at least 6 subunits, SDS-PAGE Acetobacterium woodii
7.2.2.3 hexamer 1 * 57000 + 1 * 52000 + 1 * 35000 + 1 * 19000 + 1 * 15000 + 1 * 4800, SDS-PAGE Acetobacterium woodii

Synonyms

EC Number Synonyms Comment Organism
7.2.2.1 F1FO-ATPase
-
 Acetobacterium woodii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
7.2.2.1 50 55
-
 Acetobacterium woodii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
7.2.2.1 40
-
 stable for longer periods Acetobacterium woodii
7.2.2.1 50 55 stable for 5-10 min Acetobacterium woodii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2.2.1 5.5 6 in presence of 0.1 mM Na+ Acetobacterium woodii
7.2.2.1 7 9 in presence of 5 mM Na+ Acetobacterium woodii
7.2.2.3 7 9
-
 Acetobacterium woodii