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Literature summary extracted from
- Purification and characterization of the catalytic moiety of vacuolar-type Na+-ATPase from Enterococcus hirae (1994), J. Biochem., 116, 1302-1308.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 7.2.2.1 | without glycerol, the purified enzyme, 1 mg/ml in 50 mM Tris-HCl, pH 7.5 is inactivated by overnight exposure to 4°C | Enterococcus hirae |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.2.2.1 | 7-Chloro-4-nitrobenzo-2-oxa-1,3-diazole | - |
Enterococcus hirae |  |
| 7.2.2.1 | N,N'-dicyclohexylcarbodiimide | - |
Enterococcus hirae | |
| 7.2.2.1 | NEM | - |
Enterococcus hirae | |
| 7.2.2.1 | NO3- | - |
Enterococcus hirae | |
| 7.2.2.3 | 7-Chloro-4-nitrobenzo-2-oxa-1,3-diazole | - |
Enterococcus hirae | |
| 7.2.2.3 | Dicyclohexylcarbodiimide | - |
Enterococcus hirae | |
| 7.2.2.3 | N-ethylmaleimide | - |
Enterococcus hirae | |
| 7.2.2.3 | nitrate | - |
Enterococcus hirae | |
| 7.2.2.3 | Sodium azide | - |
Enterococcus hirae | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 7.2.2.1 | membrane | - |
Enterococcus hirae | 16020 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.2.2.1 | Na+ | no stimulation by Na+ | Enterococcus hirae | |
| 7.2.2.1 | Na+ | the enzyme has Na+-translocating activity | Enterococcus hirae | |
| 7.2.2.3 | Li+ | - |
Enterococcus hirae | |
| 7.2.2.3 | Na+ | - |
Enterococcus hirae | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 7.2.2.1 | 6000 | - |
NtpA3, NtpB3, NtpD1, 3 * 6000 + 3 * 52000 + 1 * 29000, SDS-PAGE | Enterococcus hirae |
| 7.2.2.1 | 29000 | - |
NtpA3, NtpB3, NtpD1, 3 * 6000 + 3 * 52000 + 1 * 29000, SDS-PAGE | Enterococcus hirae |
| 7.2.2.1 | 52000 | - |
NtpA3, NtpB3, NtpD1, 3 * 6000 + 3 * 52000 + 1 * 29000, SDS-PAGE | Enterococcus hirae |
| 7.2.2.1 | 400000 | - |
gel filtration | Enterococcus hirae |
| 7.2.2.3 | 29000 | - |
3 * 69000 + 3 * 52000 + 1 * 29000, SDS-PAGE | Enterococcus hirae |
| 7.2.2.3 | 52000 | - |
3 * 69000 + 3 * 52000 + 1 * 29000, SDS-PAGE | Enterococcus hirae |
| 7.2.2.3 | 69000 | - |
3 * 69000 + 3 * 52000 + 1 * 29000, SDS-PAGE | Enterococcus hirae |
| 7.2.2.3 | 400000 | - |
gel filtration | Enterococcus hirae |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.2.2.3 | ATP + H2O + Na+/in | Enterococcus hirae | - |
ADP + phosphate + Na+/out | - |
r | |
| 7.2.2.3 | CTP + H2O | Enterococcus hirae | - |
? | - |
? | |
| 7.2.2.3 | GTP + H2O | Enterococcus hirae | - |
? | - |
? | |
| 7.2.2.3 | UTP + H2O | Enterococcus hirae | - |
? | - |
? | |
Organic Solvent Stability
| EC Number | Organic Solvent | Comment | Organism |
|---|---|---|---|
| 7.2.2.3 | Glycerol | - |
Enterococcus hirae |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 7.2.2.1 | Enterococcus hirae | - |
- |
- |
| 7.2.2.3 | Enterococcus hirae | - |
strain ATCC 9790 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 7.2.2.1 | - |
Enterococcus hirae |
| 7.2.2.3 | - |
Enterococcus hirae |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 7.2.2.1 | 4.73 | - |
- |
Enterococcus hirae |
| 7.2.2.3 | 4.73 | - |
- |
Enterococcus hirae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.2.2.1 | ATP + H2O | nucleotides are hydrolyzed in the order of decreasing activity: GTP, CTP, UTP, ATP | Enterococcus hirae | ADP + phosphate | - |
? | |
| 7.2.2.1 | CTP + H2O | nucleotides are hydrolyzed in the order of decreasing activity: GTP, CTP, UTP, ATP | Enterococcus hirae | CDP + H2O | - |
? | |
| 7.2.2.1 | GTP + H2O | Na+-translocating activity, GTP is hydrolyzed as the best substrate | Enterococcus hirae | GDP + phosphate | - |
? | |
| 7.2.2.1 | UTP + H2O | nucleotides are hydrolyzed in the order of decreasing activity: GTP, CTP, UTP, ATP | Enterococcus hirae | UDP + H2O | - |
? | |
| 7.2.2.3 | ATP + H2O + Na+/in | - |
Enterococcus hirae | ADP + phosphate + Na+/out | - |
r | |
| 7.2.2.3 | CTP + H2O | - |
Enterococcus hirae | ? | - |
? | |
| 7.2.2.3 | GTP + H2O | - |
Enterococcus hirae | ? | - |
? | |
| 7.2.2.3 | UTP + H2O | - |
Enterococcus hirae | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 7.2.2.1 | ? | NtpA3, NtpB3, NtpD1, 3 * 6000 + 3 * 52000 + 1 * 29000, SDS-PAGE | Enterococcus hirae |
| 7.2.2.3 | oligomer | 3 * 69000 + 3 * 52000 + 1 * 29000, SDS-PAGE | Enterococcus hirae |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 7.2.2.1 | 6 | - |
- |
Enterococcus hirae |
| 7.2.2.3 | 6 | - |
- |
Enterococcus hirae |
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