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Literature summary extracted from
- Purification and reconstitution of Na+-translocating vacuolar ATPase from Enterococcus hirae (1997), J. Biol. Chem., 272, 24885-24890.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 7.2.2.1 | - |
Enterococcus hirae |
| 7.2.2.3 | - |
Enterococcus hirae |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.2.2.1 | destruxin B | - |
Enterococcus hirae |  |
| 7.2.2.1 | N,N'-dicyclohexylcarbodiimide | - |
Enterococcus hirae | |
| 7.2.2.1 | NEM | - |
Enterococcus hirae | |
| 7.2.2.1 | NO3- | KNO3- | Enterococcus hirae | |
| 7.2.2.3 | concanamycin A | - |
Enterococcus hirae | |
| 7.2.2.3 | destruxin B | - |
Enterococcus hirae | |
| 7.2.2.3 | Dicyclohexylcarbodiimide | - |
Enterococcus hirae | |
| 7.2.2.3 | N-ethylmaleimide | - |
Enterococcus hirae | |
| 7.2.2.3 | nitrate | - |
Enterococcus hirae | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 7.2.2.1 | 0.5 | - |
ATP | - |
Enterococcus hirae | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 7.2.2.1 | plasma membrane | - |
Enterococcus hirae | 5886 | - |
| 7.2.2.3 | plasma membrane | - |
Enterococcus hirae | 5886 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.2.2.1 | Li+ | enzyme activity is strictly dependent on the presence of Na+ or Li+ ions | Enterococcus hirae | |
| 7.2.2.1 | Li+ | translocation of Li+ | Enterococcus hirae | |
| 7.2.2.1 | Na+ | the enzyme has Na+-translocating activity | Enterococcus hirae | |
| 7.2.2.1 | Na+ | enzyme activity is strictly dependent on the presence of Na+ or Li+ ions | Enterococcus hirae | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 7.2.2.1 | 8000 | - |
x * 8000 + x * 14000 + x * 15000 + x * 24000 + x * 27000 + x * 38000 + x * 52000 + x * 65000 + x * 69000, SDS-PAGE | Enterococcus hirae |
| 7.2.2.1 | 14000 | - |
x * 8000 + x * 14000 + x * 15000 + x * 24000 + x * 27000 + x * 38000 + x * 52000 + x * 65000 + x * 69000, SDS-PAGE | Enterococcus hirae |
| 7.2.2.1 | 15000 | - |
x * 8000 + x * 14000 + x * 15000 + x * 24000 + x * 27000 + x * 38000 + x * 52000 + x * 65000 + x * 69000, SDS-PAGE | Enterococcus hirae |
| 7.2.2.1 | 24000 | - |
x * 8000 + x * 14000 + x * 15000 + x * 24000 + x * 27000 + x * 38000 + x * 52000 + x * 65000 + x * 69000, SDS-PAGE | Enterococcus hirae |
| 7.2.2.1 | 27000 | - |
x * 8000 + x * 14000 + x * 15000 + x * 24000 + x * 27000 + x * 38000 + x * 52000 + x * 65000 + x * 69000, SDS-PAGE | Enterococcus hirae |
| 7.2.2.1 | 38000 | - |
x * 8000 + x * 14000 + x * 15000 + x * 24000 + x * 27000 + x * 38000 + x * 52000 + x * 65000 + x * 69000, SDS-PAGE | Enterococcus hirae |
| 7.2.2.1 | 52000 | - |
x * 8000 + x * 14000 + x * 15000 + x * 24000 + x * 27000 + x * 38000 + x * 52000 + x * 65000 + x * 69000, SDS-PAGE | Enterococcus hirae |
| 7.2.2.1 | 65000 | - |
x * 8000 + x * 14000 + x * 15000 + x * 24000 + x * 27000 + x * 38000 + x * 52000 + x * 65000 + x * 69000, SDS-PAGE | Enterococcus hirae |
| 7.2.2.1 | 69000 | - |
x * 8000 + x * 14000 + x * 15000 + x * 24000 + x * 27000 + x * 38000 + x * 52000 + x * 65000 + x * 69000, SDS-PAGE | Enterococcus hirae |
| 7.2.2.3 | 8000 | - |
1 * 69000 + 1 * 52000 + 1 * 38000 + 1 * 27000 + 1 * 24000 + 1 * 15000 + 1 * 14000 + 1 * 8000, SDS-PAGE | Enterococcus hirae |
| 7.2.2.3 | 14000 | - |
1 * 69000 + 1 * 52000 + 1 * 38000 + 1 * 27000 + 1 * 24000 + 1 * 15000 + 1 * 14000 + 1 * 8000, SDS-PAGE | Enterococcus hirae |
| 7.2.2.3 | 15000 | - |
1 * 69000 + 1 * 52000 + 1 * 38000 + 1 * 27000 + 1 * 24000 + 1 * 15000 + 1 * 14000 + 1 * 8000, SDS-PAGE | Enterococcus hirae |
| 7.2.2.3 | 24000 | - |
1 * 69000 + 1 * 52000 + 1 * 38000 + 1 * 27000 + 1 * 24000 + 1 * 15000 + 1 * 14000 + 1 * 8000, SDS-PAGE | Enterococcus hirae |
| 7.2.2.3 | 27000 | - |
1 * 69000 + 1 * 52000 + 1 * 38000 + 1 * 27000 + 1 * 24000 + 1 * 15000 + 1 * 14000 + 1 * 8000, SDS-PAGE | Enterococcus hirae |
| 7.2.2.3 | 38000 | - |
1 * 69000 + 1 * 52000 + 1 * 38000 + 1 * 27000 + 1 * 24000 + 1 * 15000 + 1 * 14000 + 1 * 8000, SDS-PAGE | Enterococcus hirae |
| 7.2.2.3 | 52000 | - |
1 * 69000 + 1 * 52000 + 1 * 38000 + 1 * 27000 + 1 * 24000 + 1 * 15000 + 1 * 14000 + 1 * 8000, SDS-PAGE | Enterococcus hirae |
| 7.2.2.3 | 69000 | - |
1 * 69000 + 1 * 52000 + 1 * 38000 + 1 * 27000 + 1 * 24000 + 1 * 15000 + 1 * 14000 + 1 * 8000, SDS-PAGE | Enterococcus hirae |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 7.2.2.1 | Enterococcus hirae | - |
- |
- |
| 7.2.2.3 | Enterococcus hirae | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 7.2.2.1 | - |
Enterococcus hirae |
| 7.2.2.3 | - |
Enterococcus hirae |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 7.2.2.1 | -20°C, solubilized enzyme is stable for at least 3 months | Enterococcus hirae |
| 7.2.2.1 | 4°C, solubilized enzyme is stable for at least 1 week | Enterococcus hirae |
| 7.2.2.3 | -80°C, n-dodecyl alpha-D-maltoside, 3 months | Enterococcus hirae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.2.2.1 | ATP + H2O | translocation of Li+ | Enterococcus hirae | ADP + phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 7.2.2.1 | ? | x * 8000 + x * 14000 + x * 15000 + x * 24000 + x * 27000 + x * 38000 + x * 52000 + x * 65000 + x * 69000, SDS-PAGE | Enterococcus hirae |
| 7.2.2.3 | oligomer | 1 * 69000 + 1 * 52000 + 1 * 38000 + 1 * 27000 + 1 * 24000 + 1 * 15000 + 1 * 14000 + 1 * 8000, SDS-PAGE | Enterococcus hirae |
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