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Literature summary extracted from

  • Nelson, N.
    Structure and function of chloroplast ATPase (1976), Biochim. Biophys. Acta, 456, 314-318.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
7.1.2.2 additional information activity is latent and must be unmasked by mild proteolysis with trypsin Spinacia oleracea

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.1.2.2 ADP
-
Euglena gracilis
7.1.2.2 ADP
-
Spinacia oleracea
7.1.2.2 Hg2+
-
Spinacia oleracea
7.1.2.2 Mg2+ 0.3 mM, 50% inhibition of Ca2+-dependent activity Spinacia oleracea
7.1.2.2 MnO4-
-
Spinacia oleracea
7.1.2.2 SO42-
-
Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.1.2.2 additional information
-
additional information
-
Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.1.2.2 chloroplast
-
Euglena gracilis 9507
-
7.1.2.2 chloroplast
-
Spinacia oleracea 9507
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.1.2.2 Ca2+
-
Euglena gracilis
7.1.2.2 Ca2+ trypsin-induced ATPase activity of solubilized CF1 is dependent on the presence of Ca2+ Spinacia oleracea
7.1.2.2 Mg2+ ATPase activity of membrane bound CF1 is Mg2+-dependent Spinacia oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.1.2.2 13000
-
x * 59000 + x * 56000 + x * 37000 + x * 17500 + x * 13000, SDS-PAGE Spinacia oleracea
7.1.2.2 17500
-
x * 59000 + x * 56000 + x * 37000 + x * 17500 + x * 13000, SDS-PAGE Spinacia oleracea
7.1.2.2 37000
-
x * 59000 + x * 56000 + x * 37000 + x * 17500 + x * 13000, SDS-PAGE Spinacia oleracea
7.1.2.2 56000
-
x * 59000 + x * 56000 + x * 37000 + x * 17500 + x * 13000, SDS-PAGE Spinacia oleracea
7.1.2.2 59000
-
x * 59000 + x * 56000 + x * 37000 + x * 17500 + x * 13000, SDS-PAGE Spinacia oleracea
7.1.2.2 325000
-
equilibrium ultracentrifugation Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
7.1.2.2 Euglena gracilis
-
-
-
7.1.2.2 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.1.2.2
-
Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.2.2 ATP + H2O + H+/in
-
Euglena gracilis ADP + phosphate + H+/out
-
?
7.1.2.2 ATP + H2O + H+/in
-
Spinacia oleracea ADP + phosphate + H+/out
-
?
7.1.2.2 GTP + H2O + H+/in
-
Euglena gracilis GDP + phosphate + H+/out
-
?
7.1.2.2 GTP + H2O + H+/in
-
Spinacia oleracea GDP + phosphate + H+/out
-
?
7.1.2.2 ITP + H2O + H+/in
-
Euglena gracilis IDP + phosphate + H+/out
-
?
7.1.2.2 ITP + H2O + H+/in
-
Spinacia oleracea IDP + phosphate + H+/out
-
?

Subunits

EC Number Subunits Comment Organism
7.1.2.2 ? x * 59000 + x * 56000 + x * 37000 + x * 17500 + x * 13000, SDS-PAGE Spinacia oleracea