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Literature summary extracted from

  • Yang, W.J.; Kim, K.W.; Lee, B.J.
    Rat brain nucleoside diphosphatase: purification and properties of type L isoenzyme (1994), Arch. Pharm. Res., 17, 256-262.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.6.1.6 ATP enhances hydrolysis of GDP Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.6 diphosphate 0.5 mM, 50% inhibition Rattus norvegicus
3.6.1.6 F- 50 mM NaF, more than 80% inhibition Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.6 1.2
-
GDP
-
Rattus norvegicus
3.6.1.6 1.4
-
UDP
-
Rattus norvegicus
3.6.1.6 1.7
-
IDP
-
Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.6 Ca2+ can partially replace Mg2+ in activation Rattus norvegicus
3.6.1.6 Mg2+ divalent cation required, maximum activity with Mg2+, half-maximal activity at 0.4 mM MgCl2 Rattus norvegicus
3.6.1.6 Mn2+ can partially replace Mg2+ in activation Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.6 45000
-
gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.6 GDP + H2O Rattus norvegicus type L enzyme chiefly works in the degradation of nucleoside diphosphates GMP + phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.6 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.6 type L isoenzyme Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.6 brain
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.6 348.4
-
-
Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.6 ADP + H2O no activity Rattus norvegicus AMP + phosphate
-
?
3.6.1.6 CDP + H2O
-
Rattus norvegicus CMP + phosphate
-
?
3.6.1.6 GDP + H2O
-
Rattus norvegicus GMP + phosphate
-
?
3.6.1.6 GDP + H2O type L enzyme chiefly works in the degradation of nucleoside diphosphates Rattus norvegicus GMP + phosphate
-
?
3.6.1.6 IDP + H2O
-
Rattus norvegicus IMP + phosphate
-
?
3.6.1.6 TDP + H2O
-
Rattus norvegicus TMP + phosphate
-
?
3.6.1.6 TPP + H2O
-
Rattus norvegicus thiamine phosphate + phosphate
-
?
3.6.1.6 UDP + H2O
-
Rattus norvegicus UMP + phosphate
-
?

Subunits

EC Number Subunits Comment Organism
3.6.1.6 monomer 1 * 45000, L-type enzyme, SDS-PAGE Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.6 6 9 hydrolysis of GDP Rattus norvegicus
3.6.1.6 7.5 8
-
Rattus norvegicus
3.6.1.6 9
-
hydrolysis of TPP Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.6.1.6 0.5
-
diphosphate
-
Rattus norvegicus